Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse - PubMed (original) (raw)

. 2005 Jan 7;307(5706):124-7.

doi: 10.1126/science.1103631.

Affiliations

• PMID: 15637282
• DOI: 10.1126/science.1103631

Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse

Takayuki Yamashita et al. Science. 2005.

Abstract

Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5'-triphosphate (GTP) analog GTPgS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

PubMed Disclaimer

Similar articles

• The role of GTP-binding protein activity in fast central synaptic transmission.
  Takahashi T, Hori T, Kajikawa Y, Tsujimoto T. Takahashi T, et al. Science. 2000 Jul 21;289(5478):460-3. doi: 10.1126/science.289.5478.460. Science. 2000. PMID: 10903208
• GTP-independent rapid and slow endocytosis at a central synapse.
  Xu J, McNeil B, Wu W, Nees D, Bai L, Wu LG. Xu J, et al. Nat Neurosci. 2008 Jan;11(1):45-53. doi: 10.1038/nn2021. Epub 2007 Dec 9. Nat Neurosci. 2008. PMID: 18066059
• Synaptic vesicle recycling at the calyx of Held.
  Xue L, Mei YA. Xue L, et al. Acta Pharmacol Sin. 2011 Mar;32(3):280-7. doi: 10.1038/aps.2010.212. Epub 2011 Jan 24. Acta Pharmacol Sin. 2011. PMID: 21258358 Free PMC article. Review.
• Ca2+-dependent regulation of synaptic vesicle endocytosis.
  Yamashita T. Yamashita T. Neurosci Res. 2012 May;73(1):1-7. doi: 10.1016/j.neures.2012.02.012. Epub 2012 Mar 3. Neurosci Res. 2012. PMID: 22401840 Review.

Cited by

• Synapsins regulate use-dependent synaptic plasticity in the calyx of Held by a Ca2+/calmodulin-dependent pathway.
  Sun J, Bronk P, Liu X, Han W, Südhof TC. Sun J, et al. Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2880-5. doi: 10.1073/pnas.0511300103. Epub 2006 Feb 15. Proc Natl Acad Sci U S A. 2006. PMID: 16481620 Free PMC article.
• AFM visualization of clathrin triskelia under fluid and in air.
  Kotova S, Prasad K, Smith PD, Lafer EM, Nossal R, Jin AJ. Kotova S, et al. FEBS Lett. 2010 Jan 4;584(1):44-8. doi: 10.1016/j.febslet.2009.11.039. FEBS Lett. 2010. PMID: 19925798 Free PMC article.
• Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules.
  Royle SJ, Lagnado L. Royle SJ, et al. Traffic. 2010 Dec;11(12):1489-97. doi: 10.1111/j.1600-0854.2010.01104.x. Traffic. 2010. PMID: 20633242 Free PMC article. Review.
• Fast neurotransmitter release regulated by the endocytic scaffold intersectin.
  Sakaba T, Kononenko NL, Bacetic J, Pechstein A, Schmoranzer J, Yao L, Barth H, Shupliakov O, Kobler O, Aktories K, Haucke V. Sakaba T, et al. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8266-71. doi: 10.1073/pnas.1219234110. Epub 2013 Apr 30. Proc Natl Acad Sci U S A. 2013. PMID: 23633571 Free PMC article.
• The kinetics of synaptic vesicle reacidification at hippocampal nerve terminals.
  Atluri PP, Ryan TA. Atluri PP, et al. J Neurosci. 2006 Feb 22;26(8):2313-20. doi: 10.1523/JNEUROSCI.4425-05.2006. J Neurosci. 2006. PMID: 16495458 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • H1 Connect