Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly - PubMed (original) (raw)

doi: 10.1038/nsmb884. Epub 2005 Jan 9.

Affiliations

PMID: 15643425
DOI: 10.1038/nsmb884

Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly

Alexandra Lusser et al. Nat Struct Mol Biol. 2005 Feb.

Abstract

CHD1 is a chromodomain-containing protein in the SNF2-like family of ATPases. Here we show that CHD1 exists predominantly as a monomer and functions as an ATP-utilizing chromatin assembly factor. This reaction involves purified CHD1, NAP1 chaperone, core histones and relaxed DNA. CHD1 catalyzes the ATP-dependent transfer of histones from the NAP1 chaperone to the DNA by a processive mechanism that yields regularly spaced nucleosomes. The comparative analysis of CHD1 and ACF revealed that CHD1 assembles chromatin with a shorter nucleosome repeat length than ACF. In addition, ACF, but not CHD1, can assemble chromatin containing histone H1, which is involved in the formation of higher-order chromatin structure and transcriptional repression. These results suggest a role for CHD1 in the assembly of active chromatin and a function of ACF in the assembly of repressive chromatin.

PubMed Disclaimer

Cited by

Ruler elements in chromatin remodelers set nucleosome array spacing and phasing.
Oberbeckmann E, Niebauer V, Watanabe S, Farnung L, Moldt M, Schmid A, Cramer P, Peterson CL, Eustermann S, Hopfner KP, Korber P. Oberbeckmann E, et al. Nat Commun. 2021 May 28;12(1):3232. doi: 10.1038/s41467-021-23015-0. Nat Commun. 2021. PMID: 34050140 Free PMC article.
The basic linker of macroH2A stabilizes DNA at the entry/exit site of the nucleosome.
Chakravarthy S, Patel A, Bowman GD. Chakravarthy S, et al. Nucleic Acids Res. 2012 Sep 1;40(17):8285-95. doi: 10.1093/nar/gks645. Epub 2012 Jun 29. Nucleic Acids Res. 2012. PMID: 22753032 Free PMC article.
Neuronal transcription of autism gene PTCHD1 is regulated by a conserved downstream enhancer sequence.
Pastore SF, Muhammad T, Stan C, Frankland PW, Hamel PA, Vincent JB. Pastore SF, et al. Sci Rep. 2023 Nov 21;13(1):20391. doi: 10.1038/s41598-023-46673-0. Sci Rep. 2023. PMID: 37990104 Free PMC article.
Establishment of a promoter-based chromatin architecture on recently replicated DNA can accommodate variable inter-nucleosome spacing.
Fennessy RT, Owen-Hughes T. Fennessy RT, et al. Nucleic Acids Res. 2016 Sep 6;44(15):7189-203. doi: 10.1093/nar/gkw331. Epub 2016 Apr 22. Nucleic Acids Res. 2016. PMID: 27106059 Free PMC article.
Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family.
Shukla MS, Syed SH, Boopathi R, Simon EB, Nahata S, Ramos L, Dalkara D, Moskalenko C, Travers A, Angelov D, Dimitrov S, Hamiche A, Bednar J. Shukla MS, et al. Sci Rep. 2019 Oct 2;9(1):14212. doi: 10.1038/s41598-019-50572-8. Sci Rep. 2019. PMID: 31578361 Free PMC article.

Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly - PubMed (original) (raw)