Bacterial virus phi29 DNA-packaging motor and its potential applications in gene therapy and nanotechnology - PubMed (original) (raw)

Review

Bacterial virus phi29 DNA-packaging motor and its potential applications in gene therapy and nanotechnology

Peixuan Guo. Methods Mol Biol. 2005.

Abstract

A controllable, 30-nm imitating DNA-packaging motor was constructed. The motor is driven by six synthetic adenosine triphosphate (ATP)-binding RNA (packaging RNA [pRNA]) monomers, similar to the driving of a bolt with a hex nut. Conformational change and sequential action of the RNA with fivefold (viral capsid)/sixfold (pRNA hexamer) mismatch could ensure continuous rotation of the motor with ATP as energy. In the presence of ATP and magnesium, a 5-microm synthetic DNA was packaged using this motor. On average, one ATP was used to translocate two bases of DNA. The DNA-filled capsids were subsequently converted into up to 109 PFU/mL of infectious virus. The three-dimensional structures of pRNA monomer, dimer, and hexamer have been probed by photoaffinity crosslinking, chemical modification interference, cryo-atomic force microscopy, and computer modeling. The pRNA's size and shape can be controlled and manipulated at will to form stable dimers and trimers. Cryo-atomic force microscopy revealed that monomers, dimers, and trimers displayed a checkmark outline, elongated shape, and triangular structure, respectively. The motor can be turned off by gamma-S-ATP or EDTA and turned on again with the addition of ATP or magnesium, respectively. The formation of ordered structural arrays of the motor complex and its components, the retention of motor function after the 3'-end extension of the pRNA, and the ease of RNA dimer, trimer, and hexamer manipulation with desired shape and size make this RNA-containing motor a promising tool for drug and gene delivery and for use in nanodevices.

PubMed Disclaimer

Cited by

Recent Advances in miRNA Delivery Systems.
Dasgupta I, Chatterjee A. Dasgupta I, et al. Methods Protoc. 2021 Jan 20;4(1):10. doi: 10.3390/mps4010010. Methods Protoc. 2021. PMID: 33498244 Free PMC article.
ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor.
Cai R, Price IR, Ding F, Wu F, Chen T, Zhang Y, Liu G, Jardine PJ, Lu C, Ke A. Cai R, et al. Nucleic Acids Res. 2019 Oct 10;47(18):9818-9828. doi: 10.1093/nar/gkz692. Nucleic Acids Res. 2019. PMID: 31396619 Free PMC article.
Self-Assembly of Tail Tube Protein of Bacteriophage vB_EcoS_NBD2 into Extremely Long Polytubes in E. coli and S. cerevisiae.
Špakova A, Šimoliūnas E, Batiuškaitė R, Pajeda S, Meškys R, Petraitytė-Burneikienė R. Špakova A, et al. Viruses. 2019 Mar 1;11(3):208. doi: 10.3390/v11030208. Viruses. 2019. PMID: 30832262 Free PMC article.
The Robust Self-Assembling Tubular Nanostructures Formed by gp053 from Phage vB_EcoM_FV3.
Šimoliūnas E, Truncaitė L, Rutkienė R, Povilonienė S, Goda K, Kaupinis A, Valius M, Meškys R. Šimoliūnas E, et al. Viruses. 2019 Jan 11;11(1):50. doi: 10.3390/v11010050. Viruses. 2019. PMID: 30641882 Free PMC article.
Thermodynamic stabilities of three-way junction nanomotifs in prohead RNA.
Hill AC, Schroeder SJ. Hill AC, et al. RNA. 2017 Apr;23(4):521-529. doi: 10.1261/rna.059220.116. Epub 2017 Jan 9. RNA. 2017. PMID: 28069889 Free PMC article.

Bacterial virus phi29 DNA-packaging motor and its potential applications in gene therapy and nanotechnology - PubMed (original) (raw)