Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae - PubMed (original) (raw)

. 2005 May;61(5):499-507.

doi: 10.1002/ps.1001.

Affiliations

• PMID: 15657956
• DOI: 10.1002/ps.1001

Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae

Thomas Van Leeuwen et al. Pest Manag Sci. 2005 May.

Abstract

A field-collected strain (MR-VL) of the two-spotted spider mite, Tetranychus urticae Koch, exhibited strong resistance to bifenthrin, dicofol and fenbutatin oxide in comparison with a susceptible laboratory strain (LS-VL). The MR-VL strain was screened for cross-resistance with several currently used acaricides. Cross-resistance was detected with clofentezine (RR = 2631), dimethoate (RR = 250), chlorfenapyr (RR = 154), bromopropylate (RR = 25), amitraz (RR = 17), flucycloxuron (RR = 15) and azocyclotin (RR = 7). Abamectin, acequinocyl, bifenazate, tebufenpyrad and spirodiclofen did not show any signs of cross-resistance. Enhanced detoxification by increased activity of mono-oxygenases (MO) and esterases is at least partially responsible for the observed resistance and cross-resistance. MO assays with 7-ethoxycoumarin (7-EC) were optimised and 7-ethoxy-4-trifluoromethylcoumarin (7-EFC), a new MO-substrate, was evaluated for the first time in T urticae and proved to be a good alternative to 7-EC. Approximately 3- and 4-fold higher MO activity was detected with 7-EFC and 7-EC respectively in the MR-VL strain. Kinetic parameters of general esterase assays with 4-nitrophenyl acetate and 1-naphthyl acetate as substrate indicated that more esterases were present in the MR-VL strain. A first attempt was made to classify the esterases present in T urticae. Acetyl-, aryl- and carboxyl-esterases were detected with the use of inhibitors after separation by native PAGE. Glutathione-S-transferases did not seem to play any role in the observed resistance and no differences were detected when the general oxidative capacities of the two strains were compared.

PubMed Disclaimer

Similar articles

• Fenpyroximate resistance in Tetranychus urticae (Acari: Tetranychidae): cross-resistance and biochemical resistance mechanisms.
  Kim YJ, Lee SH, Lee SW, Ahn YJ. Kim YJ, et al. Pest Manag Sci. 2004 Oct;60(10):1001-6. doi: 10.1002/ps.909. Pest Manag Sci. 2004. PMID: 15481826
• Biochemical analysis of a chlorfenapyr-selected resistant strain of Tetranychus urticae Koch.
  Van Leeuwen T, Van Pottelberge S, Tirry L. Van Leeuwen T, et al. Pest Manag Sci. 2006 May;62(5):425-33. doi: 10.1002/ps.1183. Pest Manag Sci. 2006. PMID: 16550506
• Esterase-mediated bifenthrin resistance in a multiresistant strain of the two-spotted spider mite, Tetranychus urticae.
  Van Leeuwen T, Tirry L. Van Leeuwen T, et al. Pest Manag Sci. 2007 Feb;63(2):150-6. doi: 10.1002/ps.1314. Pest Manag Sci. 2007. PMID: 17125154
• Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: a review.
  Van Leeuwen T, Vontas J, Tsagkarakou A, Dermauw W, Tirry L. Van Leeuwen T, et al. Insect Biochem Mol Biol. 2010 Aug;40(8):563-72. doi: 10.1016/j.ibmb.2010.05.008. Epub 2010 Jun 8. Insect Biochem Mol Biol. 2010. PMID: 20685616 Review.
• Toxicodynamic mechanisms and monitoring of acaricide resistance in the two-spotted spider mite.
  Kwon DH, Clark JM, Lee SH. Kwon DH, et al. Pestic Biochem Physiol. 2015 Jun;121:97-101. doi: 10.1016/j.pestbp.2014.12.011. Epub 2014 Dec 15. Pestic Biochem Physiol. 2015. PMID: 26047116 Review.

Cited by

• A Comprehensive Review of the Current Knowledge of Chlorfenapyr: Synthesis, Mode of Action, Resistance, and Environmental Toxicology.
  Huang P, Yan X, Yu B, He X, Lu L, Ren Y. Huang P, et al. Molecules. 2023 Nov 20;28(22):7673. doi: 10.3390/molecules28227673. Molecules. 2023. PMID: 38005396 Free PMC article. Review.
• A glutamate-gated chloride channel as the mite-specific target-site of dicofol and other diphenylcarbinol acaricides.
  Vandenhole M, Mermans C, De Beer B, Xue W, Zhao Y, Ozoe Y, Liu G, Dermauw W, Van Leeuwen T. Vandenhole M, et al. Commun Biol. 2023 Nov 13;6(1):1160. doi: 10.1038/s42003-023-05488-5. Commun Biol. 2023. PMID: 37957415 Free PMC article.
• Toxicity and Control Efficacy of an Organosilicone to the Two-Spotted Spider Mite Tetranychus urticae and Its Crop Hosts.
  Chen JC, Ma ZZ, Gong YJ, Cao LJ, Wang JX, Guo SK, Hoffmann AA, Wei SJ. Chen JC, et al. Insects. 2022 Mar 30;13(4):341. doi: 10.3390/insects13040341. Insects. 2022. PMID: 35447783 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley