Intestinal dendritic cell subsets: differential effects of systemic TLR4 stimulation on migratory fate and activation in vivo - PubMed (original) (raw)
Comparative Study
. 2005 Feb 1;174(3):1374-84.
doi: 10.4049/jimmunol.174.3.1374.
Affiliations
- PMID: 15661895
- DOI: 10.4049/jimmunol.174.3.1374
Comparative Study
Intestinal dendritic cell subsets: differential effects of systemic TLR4 stimulation on migratory fate and activation in vivo
Emma L Turnbull et al. J Immunol. 2005.
Abstract
Dendritic cells (DC) present peripheral Ags to T cells in lymph nodes, but also influence their differentiation (tolerance/immunity, Th1/Th2). To investigate how peripheral conditions affect DC properties and might subsequently regulate T cell differentiation, we examined the effects of a potent DC-activating, TLR-4-mediated stimulus, LPS, on rat intestinal and hepatic DC in vivo. Steady-state rat intestinal and hepatic lymph DC are alpha(E2) integrin(high) (CD103) and include two subsets, signal regulatory protein alpha (SIRPalpha)(hi/low), probably representing murine CD8alphaalpha(-/+) DC. Steady-state lamina propria DC are immature; surface MHC class II(low), but steady-state lymph DC are semimature, MHC class II(high), but CD80/86(low). Intravenous LPS induced rapid lamina propria DC emigration and increased lymph DC traffic without altering SIRPalpha(high)/SIRPalpha(low) proportions. CD80/86 expression on lymph or mesenteric node DC was not up-regulated after i.v. LPS. In contrast, i.v. LPS stimulated marked CD80/86 up-regulation on splenic DC. CD80/86 expression on intestinal lymph DC, however, was increased after in vitro culture with TNF-alpha or GM-CSF, but not with up to 5 mug/ml LPS. Steady-state SIRPalpha(low) DC localized to T cell areas of mesenteric nodes, spleen, and Peyer's patch, whereas SIRPalpha(high) DC were excluded from these areas. Intravenous LPS stimulated rapid and abundant SIRPalpha(high) DC accumulation in T cell areas of mesenteric nodes and spleen. In striking contrast, i.v. LPS had no effect on DC numbers or distribution in Peyer's patches. Our results suggest that any explanation of switching between tolerance and immunity as well as involving changes in DC activation status must also take into account differential migration of DC subsets.
Similar articles
- Site-specific expression of CD11b and SIRPalpha (CD172a) on dendritic cells: implications for their migration patterns in the gut immune system.
Bimczok D, Sowa EN, Faber-Zuschratter H, Pabst R, Rothkötter HJ. Bimczok D, et al. Eur J Immunol. 2005 May;35(5):1418-27. doi: 10.1002/eji.200425726. Eur J Immunol. 2005. PMID: 15827962 - Rat intestinal dendritic cells: immunostimulatory potency and phenotypic characterization.
Liu LM, MacPherson GG. Liu LM, et al. Immunology. 1995 May;85(1):88-93. Immunology. 1995. PMID: 7635526 Free PMC article. - Regulation of mucosal dendritic cell function by receptor activator of NF-kappa B (RANK)/RANK ligand interactions: impact on tolerance induction.
Williamson E, Bilsborough JM, Viney JL. Williamson E, et al. J Immunol. 2002 Oct 1;169(7):3606-12. doi: 10.4049/jimmunol.169.7.3606. J Immunol. 2002. PMID: 12244151 - Phenotype and function of rat dendritic cell subsets.
Yrlid U, Macpherson G. Yrlid U, et al. APMIS. 2003 Jul-Aug;111(7-8):756-65. doi: 10.1034/j.1600-0463.2003.11107807.x. APMIS. 2003. PMID: 12974777 Review. - Subsets of migrating intestinal dendritic cells.
Milling S, Yrlid U, Cerovic V, MacPherson G. Milling S, et al. Immunol Rev. 2010 Mar;234(1):259-67. doi: 10.1111/j.0105-2896.2009.00866.x. Immunol Rev. 2010. PMID: 20193024 Review.
Cited by
- The in vitro gastrointestinal digestion-associated protein corona of polystyrene nano- and microplastics increases their uptake by human THP-1-derived macrophages.
Brouwer H, Porbahaie M, Boeren S, Busch M, Bouwmeester H. Brouwer H, et al. Part Fibre Toxicol. 2024 Feb 4;21(1):4. doi: 10.1186/s12989-024-00563-z. Part Fibre Toxicol. 2024. PMID: 38311718 Free PMC article. - (Not) Home alone: Antigen presenting cell - T Cell communication in barrier tissues.
Neuwirth T, Knapp K, Stary G. Neuwirth T, et al. Front Immunol. 2022 Sep 29;13:984356. doi: 10.3389/fimmu.2022.984356. eCollection 2022. Front Immunol. 2022. PMID: 36248804 Free PMC article. Review. - Modulation of Gut Microbiota by Magnesium Isoglycyrrhizinate Mediates Enhancement of Intestinal Barrier Function and Amelioration of Methotrexate-Induced Liver Injury.
Xia Y, Shi H, Qian C, Han H, Lu K, Tao R, Gu R, Zhao Y, Wei Z, Lu Y. Xia Y, et al. Front Immunol. 2022 May 12;13:874878. doi: 10.3389/fimmu.2022.874878. eCollection 2022. Front Immunol. 2022. PMID: 35634319 Free PMC article. - Impact of the Microbiome on the Immune System.
Lambring CB, Siraj S, Patel K, Sankpal UT, Mathew S, Basha R. Lambring CB, et al. Crit Rev Immunol. 2019;39(5):313-328. doi: 10.1615/CritRevImmunol.2019033233. Crit Rev Immunol. 2019. PMID: 32422014 Free PMC article. Review. - Intestinal Dendritic Cells in Health and Gut Inflammation.
Stagg AJ. Stagg AJ. Front Immunol. 2018 Dec 6;9:2883. doi: 10.3389/fimmu.2018.02883. eCollection 2018. Front Immunol. 2018. PMID: 30574151 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous