Role of Krüppel-like factor 15 (KLF15) in transcriptional regulation of adipogenesis - PubMed (original) (raw)
. 2005 Apr 1;280(13):12867-75.
doi: 10.1074/jbc.M410515200. Epub 2005 Jan 20.
Hiroshi Sakaue, Haruhisa Iguchi, Hideyuki Gomi, Yuko Okada, Yasuhiro Takashima, Kyoko Nakamura, Takehiro Nakamura, Toshimasa Yamauchi, Naoto Kubota, Takashi Kadowaki, Yasushi Matsuki, Wataru Ogawa, Ryuji Hiramatsu, Masato Kasuga
Affiliations
- PMID: 15664998
- DOI: 10.1074/jbc.M410515200
Free article
Role of Krüppel-like factor 15 (KLF15) in transcriptional regulation of adipogenesis
Toshiyuki Mori et al. J Biol Chem. 2005.
Free article
Abstract
Krüppel-like zinc finger transcription factors (KLFs) play diverse roles during cell differentiation and development in mammals. We have now shown by microarray analysis that expression of the KLF15 gene is markedly up-regulated during the differentiation of 3T3-L1 preadipocytes into adipocytes. Inhibition of the function of KLF15, either by expression of a dominant negative mutant or by RNA interference, both reduced the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and blocked adipogenesis in 3T3-L1 preadipocytes exposed to inducers of adipocyte differentiation. However, the dominant negative mutant of KLF15 did not affect the expression of CCAAT/enhancer-binding protein beta (C/EBPbeta) elicited by inducers of differentiation in 3T3-L1 preadipocytes. In addition, ectopic expression of KLF15 in NIH 3T3 or C2C12 cells triggered both lipid accumulation and the expression of PPARgamma in the presence of inducers of adipocyte differentiation. Ectopic expression of C/EBPbeta, C/EBPdelta, or C/EBPalpha in NIH 3T3 cells also elicited the expression of KLF15 in the presence of inducers of adipocyte differentiation. Moreover, KLF15 and C/EBPalpha acted synergistically to increase the activity of the PPARgamma2 gene promoter in 3T3-L1 adipocytes. Our observations thus demonstrate that KLF15 plays an essential role in adipogenesis in 3T3-L1 cells through its regulation of PPAR gamma expression.
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