Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat - PubMed (original) (raw)
Comparative Study
doi: 10.1159/000083140. Epub 2005 Jan 5.
Affiliations
- PMID: 15665556
- DOI: 10.1159/000083140
Comparative Study
Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat
Michael S Irwig et al. Neuroendocrinology. 2004.
Abstract
The KiSS-1 gene codes for a family of neuropeptides called kisspeptins which bind to the G-protein-coupled receptor GPR54. To assess the possible effects of kisspeptins on gonadotropin secretion, we injected kisspeptin-52 into the lateral cerebral ventricles of adult male rats and found that kisspeptin-52 increased the serum levels of luteinizing hormone (p < 0.05). To determine whether the kisspeptin-52-induced stimulation of luteinizing hormone secretion was mediated by gonadotropin-releasing hormone (GnRH), we pretreated adult male rats with a GnRH antagonist (acyline), then challenged the animals with intracerebroventricularly administered kisspeptin-52. The GnRH antagonist blocked the kisspeptin-52-induced increase in luteinizing hormone. To examine whether kisspeptins stimulate transcriptional activity in GnRH neurons, we administered kisspeptin-52 intracerebroventricularly and found by immunocytochemistry that 86% of the GnRH neurons coexpressed Fos 2 h after the kisspeptin-52 challenge, whereas fewer than 1% of the GnRH neurons expressed Fos following injection of the vehicle alone (p < 0.001). To assess whether kisspeptins can directly act on GnRH neurons, we used double-label in situ hybridization and found that 77% of the GnRH neurons coexpress GPR54 mRNA. Finally, to determine whether KiSS-1 gene expression is regulated by gonadal hormones, we measured KiSS-1 mRNA levels by single-label in situ hybridization in intact and castrated males and found significantly higher levels in the arcuate nucleus of castrates. These results demonstrate that GnRH neurons are direct targets for regulation by kisspeptins and that KiSS-1 mRNA is regulated by gonadal hormones, suggesting that KiSS-1 neurons play an important role in the feedback regulation of gonadotropin secretion.
Similar articles
- A role for kisspeptins in the regulation of gonadotropin secretion in the mouse.
Gottsch ML, Cunningham MJ, Smith JT, Popa SM, Acohido BV, Crowley WF, Seminara S, Clifton DK, Steiner RA. Gottsch ML, et al. Endocrinology. 2004 Sep;145(9):4073-7. doi: 10.1210/en.2004-0431. Epub 2004 Jun 24. Endocrinology. 2004. PMID: 15217982 - The role of kisspeptin-GPR54 signaling in the tonic regulation and surge release of gonadotropin-releasing hormone/luteinizing hormone.
Dungan HM, Gottsch ML, Zeng H, Gragerov A, Bergmann JE, Vassilatis DK, Clifton DK, Steiner RA. Dungan HM, et al. J Neurosci. 2007 Oct 31;27(44):12088-95. doi: 10.1523/JNEUROSCI.2748-07.2007. J Neurosci. 2007. PMID: 17978050 Free PMC article. - KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo by oestradiol and gonadotrophin-releasing hormone.
Richard N, Galmiche G, Corvaisier S, Caraty A, Kottler ML. Richard N, et al. J Neuroendocrinol. 2008 Mar;20(3):381-93. doi: 10.1111/j.1365-2826.2008.01653.x. Epub 2008 Jan 17. J Neuroendocrinol. 2008. PMID: 18208554 - Minireview: kisspeptin neurons as central processors in the regulation of gonadotropin-releasing hormone secretion.
Dungan HM, Clifton DK, Steiner RA. Dungan HM, et al. Endocrinology. 2006 Mar;147(3):1154-8. doi: 10.1210/en.2005-1282. Epub 2005 Dec 22. Endocrinology. 2006. PMID: 16373418 Review. - Kisspeptin signalling in the brain: steroid regulation in the rodent and ewe.
Smith JT. Smith JT. Brain Res Rev. 2008 Mar;57(2):288-98. doi: 10.1016/j.brainresrev.2007.04.002. Epub 2007 Apr 19. Brain Res Rev. 2008. PMID: 17509691 Review.
Cited by
- Androgen Inhibition of Reproductive Neuroendocrine Function in Females and Transgender Males.
Kauffman AS. Kauffman AS. Endocrinology. 2024 Aug 27;165(10):bqae113. doi: 10.1210/endocr/bqae113. Endocrinology. 2024. PMID: 39207217 Review. - Chronic inflammation decreases arcuate kisspeptin expression in male sheep.
Renwick AN, Whitlock BK, Nestor CC, Daniel JA, Strickland L, Lear AS, Adkins M, Griffin C, Esteller-Vico A. Renwick AN, et al. Domest Anim Endocrinol. 2024 Oct;89:106868. doi: 10.1016/j.domaniend.2024.106868. Epub 2024 Jun 9. Domest Anim Endocrinol. 2024. PMID: 38901139 - Kisspeptin signaling in astrocytes modulates the reproductive axis.
Torres E, Pellegrino G, Granados-Rodríguez M, Fuentes-Fayos AC, Velasco I, Coutteau-Robles A, Legrand A, Shanabrough M, Perdices-Lopez C, Leon S, Yeo SH, Manchishi SM, Sánchez-Tapia MJ, Navarro VM, Pineda R, Roa J, Naftolin F, Argente J, Luque RM, Chowen JA, Horvath TL, Prevot V, Sharif A, Colledge WH, Tena-Sempere M, Romero-Ruiz A. Torres E, et al. J Clin Invest. 2024 Jun 11;134(15):e172908. doi: 10.1172/JCI172908. J Clin Invest. 2024. PMID: 38861336 Free PMC article. - Impact of stress on male fertility: role of gonadotropin inhibitory hormone.
Odetayo AF, Akhigbe RE, Bassey GE, Hamed MA, Olayaki LA. Odetayo AF, et al. Front Endocrinol (Lausanne). 2024 Jan 8;14:1329564. doi: 10.3389/fendo.2023.1329564. eCollection 2023. Front Endocrinol (Lausanne). 2024. PMID: 38260147 Free PMC article. Review. - Is There an Association Between Circulating Kisspeptin Levels and Ovarian Reserve in Women of Reproductive Age?
Nikolettos K, Vlahos N, Pagonopoulou O, Nikolettos N, Zikopoulos K, Tsikouras P, Kontomanolis E, Damaskos C, Garmpis N, Asimakopoulos B. Nikolettos K, et al. In Vivo. 2023 Sep-Oct;37(5):2219-2223. doi: 10.21873/invivo.13322. In Vivo. 2023. PMID: 37652519 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases