Cre-mediated recombination in cell lineages that express the progesterone receptor - PubMed (original) (raw)
Cre-mediated recombination in cell lineages that express the progesterone receptor
Selma M Soyal et al. Genesis. 2005 Feb.
Abstract
Using gene-targeting methods, a progesterone receptor Cre knockin (PR-Cre) mouse was generated in which Cre recombinase was inserted into exon 1 of the PR gene. The insertion positions the Cre gene downstream (and under the specific control) of the endogenous PR promoter. As for heterozygotes for the progesterone receptor knockout (PRKO) mutation, mice heterozygous for the Cre knockin insertion are phenotypically indistinguishable from wildtype. Crossing the PR-Cre with the ROSA26R reporter revealed that Cre excision activity is restricted to cells that express PR in progesterone-responsive tissues such as the uterus, ovary, oviduct, pituitary gland, and mammary gland. Initial characterization of the PR-Cre mouse underscores the utility of this model to precisely ablate floxed target genes specifically in cell lineages that express the PR. In the wider context of female reproductive tissue ontology, this model will be indispensable in tracing the developmental fate of cell lineages that descend from PR positive progenitors.
Copyright (c) 2005 Wiley-Liss, Inc.
Similar articles
- Generation of a mouse for conditional excision of progesterone receptor.
Hashimoto-Partyka MK, Lydon JP, Iruela-Arispe ML. Hashimoto-Partyka MK, et al. Genesis. 2006 Aug;44(8):391-5. doi: 10.1002/dvg.20227. Genesis. 2006. PMID: 16868919 - Targeting iCre expression to murine progesterone receptor cell-lineages using bacterial artificial chromosome transgenesis.
Mukherjee A, Soyal SM, Wheeler DA, Fernandez-Valdivia R, Nguyen J, DeMayo FJ, Lydon JP. Mukherjee A, et al. Genesis. 2006 Dec;44(12):601-10. doi: 10.1002/dvg.20257. Genesis. 2006. PMID: 17149722 - Reproductive tissue selective actions of progesterone receptors.
Mulac-Jericevic B, Conneely OM. Mulac-Jericevic B, et al. Reproduction. 2004 Aug;128(2):139-46. doi: 10.1530/rep.1.00189. Reproduction. 2004. PMID: 15280552 Review. - Gene transfer and genetic modification of embryonic stem cells by Cre- and Cre-PR-expressing MESV-based retroviral vectors.
Psarras S, Karagianni N, Kellendonk C, Tronche F, Cosset FL, Stocking C, Schirrmacher V, Boehmer Hv Hv, Khazaie K. Psarras S, et al. J Gene Med. 2004 Jan;6(1):32-42. doi: 10.1002/jgm.442. J Gene Med. 2004. PMID: 14716675 - Conditional gene targeting in the mouse nervous system: Insights into brain function and diseases.
Gavériaux-Ruff C, Kieffer BL. Gavériaux-Ruff C, et al. Pharmacol Ther. 2007 Mar;113(3):619-34. doi: 10.1016/j.pharmthera.2006.12.003. Epub 2007 Jan 10. Pharmacol Ther. 2007. PMID: 17289150 Review.
Cited by
- Uterine development and fertility are dependent on gene dosage of the nuclear receptor coregulator REA.
Park S, Yoon S, Zhao Y, Park SE, Liao L, Xu J, Lydon JP, DeMayo FJ, O'Malley BW, Bagchi MK, Katzenellenbogen BS. Park S, et al. Endocrinology. 2012 Aug;153(8):3982-94. doi: 10.1210/en.2012-1044. Epub 2012 May 14. Endocrinology. 2012. PMID: 22585830 Free PMC article. - Spatiotemporally distinct roles of cyclooxygenase-1 and cyclooxygenase-2 at fetomaternal interface in mice.
Aikawa S, Matsuo M, Akaeda S, Sugimoto Y, Arita M, Isobe Y, Sugiura Y, Taira S, Maeda R, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Fukui Y, Hiraoka T, Harada M, Wada-Hiraike O, Osuga Y, Hirota Y. Aikawa S, et al. JCI Insight. 2024 Aug 27;9(19):e181865. doi: 10.1172/jci.insight.181865. JCI Insight. 2024. PMID: 39377223 Free PMC article. - Roles of lipid mediators in early pregnancy events.
Aikawa S, Hirota Y. Aikawa S, et al. Reprod Med Biol. 2024 Jul 15;23(1):e12597. doi: 10.1002/rmb2.12597. eCollection 2024 Jan-Dec. Reprod Med Biol. 2024. PMID: 39010880 Free PMC article. Review. - Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice.
Tu Z, Wang Q, Cui T, Wang J, Ran H, Bao H, Lu J, Wang B, Lydon JP, DeMayo F, Zhang S, Kong S, Wu X, Wang H. Tu Z, et al. Cell Death Differ. 2016 Jan;23(1):169-81. doi: 10.1038/cdd.2015.98. Epub 2015 Jul 17. Cell Death Differ. 2016. PMID: 26184908 Free PMC article. - Establishing 3D Endometrial Organoids from the Mouse Uterus.
Tang S, Parks SE, Liao Z, Cope DI, Blutt SE, Monsivais D. Tang S, et al. J Vis Exp. 2023 Jan 6;(191):10.3791/64448. doi: 10.3791/64448. J Vis Exp. 2023. PMID: 36688555 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials