Sec61p and BiP directly facilitate polypeptide translocation into the ER - PubMed (original) (raw)
Sec61p and BiP directly facilitate polypeptide translocation into the ER
S L Sanders et al. Cell. 1992.
Abstract
Secretory proteins are segregated from cytosolic proteins by their translocation into the endoplasmic reticulum (ER). A modified secretory protein trapped during translocation across the ER membrane can be crosslinked to two previously identified proteins, Sec61p and BiP (Kar2p). The dependence of this cross-linking upon proteins and small molecules was examined. Mutations in SEC62 and SEC63 decrease the ability of Sec61p to be cross-linked to the secretory polypeptide trapped in translocation. ATP is also required for interaction of Sec61p with the secretory protein. Three kar2 alleles display defective translocation in vitro. Two of these alleles also decrease the ability of Sec61p to be cross-linked to the secretory protein. The third allele, while exhibiting a severe translocation defect, does not affect the interaction of Sec61p with the secretory protein. These results suggest that Sec61p is directly involved in translocation and that BiP acts at two stages of the translocation cycle.
Similar articles
- Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae.
Lyman SK, Schekman R. Lyman SK, et al. J Cell Biol. 1995 Dec;131(5):1163-71. doi: 10.1083/jcb.131.5.1163. J Cell Biol. 1995. PMID: 8522580 Free PMC article. - Sec61p serves multiple roles in secretory precursor binding and translocation into the endoplasmic reticulum membrane.
Pilon M, Römisch K, Quach D, Schekman R. Pilon M, et al. Mol Biol Cell. 1998 Dec;9(12):3455-73. doi: 10.1091/mbc.9.12.3455. Mol Biol Cell. 1998. PMID: 9843581 Free PMC article. - Sec61p mediates export of a misfolded secretory protein from the endoplasmic reticulum to the cytosol for degradation.
Pilon M, Schekman R, Römisch K. Pilon M, et al. EMBO J. 1997 Aug 1;16(15):4540-8. doi: 10.1093/emboj/16.15.4540. EMBO J. 1997. PMID: 9303298 Free PMC article. - Polypeptide translocation machinery of the yeast endoplasmic reticulum.
Lyman SK, Schekman R. Lyman SK, et al. Experientia. 1996 Dec 15;52(12):1042-9. doi: 10.1007/BF01952100. Experientia. 1996. PMID: 8988244 Review. - Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation.
Jung SJ, Kim H. Jung SJ, et al. Int J Mol Sci. 2021 Nov 25;22(23):12757. doi: 10.3390/ijms222312757. Int J Mol Sci. 2021. PMID: 34884562 Free PMC article. Review.
Cited by
- Comparative Transcriptome Analysis of Head Kidney of Aeromonas hydrophila-infected Hypoxia-tolerant and Normal Large Yellow Croaker.
Zhang Y, Shen W, Ding J, Gao X, Wu X, Zhu J. Zhang Y, et al. Mar Biotechnol (NY). 2022 Dec;24(6):1039-1054. doi: 10.1007/s10126-022-10158-4. Epub 2022 Sep 21. Mar Biotechnol (NY). 2022. PMID: 36129638 - Take Me Home, Protein Roads: Structural Insights into Signal Peptide Interactions during ER Translocation.
Liaci AM, Förster F. Liaci AM, et al. Int J Mol Sci. 2021 Nov 1;22(21):11871. doi: 10.3390/ijms222111871. Int J Mol Sci. 2021. PMID: 34769302 Free PMC article. Review. - Chaperone-mediated reflux of secretory proteins to the cytosol during endoplasmic reticulum stress.
Igbaria A, Merksamer PI, Trusina A, Tilahun F, Johnson JR, Brandman O, Krogan NJ, Weissman JS, Papa FR. Igbaria A, et al. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11291-11298. doi: 10.1073/pnas.1904516116. Epub 2019 May 17. Proc Natl Acad Sci U S A. 2019. PMID: 31101715 Free PMC article. - The Emerging Roles of Early Protein Folding Events in the Secretory Pathway in the Development of Neurodegenerative Maladies.
Dubnikov T, Cohen E. Dubnikov T, et al. Front Neurosci. 2017 Feb 7;11:48. doi: 10.3389/fnins.2017.00048. eCollection 2017. Front Neurosci. 2017. PMID: 28223916 Free PMC article. Review. - Protein Quality Control in Health and Disease.
Dubnikov T, Ben-Gedalya T, Cohen E. Dubnikov T, et al. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a023523. doi: 10.1101/cshperspect.a023523. Cold Spring Harb Perspect Biol. 2017. PMID: 27864315 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases