Extrathymic hemopoietic progenitors committed to T cell differentiation in the adult mouse - PubMed (original) (raw)
Comparative Study
. 2005 Feb 15;174(4):1980-8.
doi: 10.4049/jimmunol.174.4.1980.
Affiliations
- PMID: 15699126
- DOI: 10.4049/jimmunol.174.4.1980
Comparative Study
Extrathymic hemopoietic progenitors committed to T cell differentiation in the adult mouse
Marie-Laure Arcangeli et al. J Immunol. 2005.
Abstract
The role of the thymus in T cell commitment of hemopoietic precursor is yet controversial. We previously identified a major T cell progenitor activity in precursor cells isolated from bone marrow-derived spleen colonies. In this study, we characterize the properties of these pre-T cells. We demonstrate that they have unique phenotype and can be generated in a total absence of any thymic influence. Indeed, even when studied at the single-cell level, extrathymic T cell-committed precursors express T cell-specific genes. Moreover, these cells are not committed to a particular T cell differentiation pathway because they can generate both extrathymic CD8alphaalpha+ intraepithelial lymphocytes and thymus-derived conventional thymocytes. We also compared these pre-T cells with fully T cell-committed thymic progenitors. When tested in vitro or by direct intrathymic transfer, these cells have a low clonogenic activity. However, after i.v. transfer, thymus repopulation is efficient and these precursors generate very high numbers of peripheral T cells. These results suggest the existence of extra steps of pre-T cell maturation that improve thymus reconstitution capacity and that can be delivered even after full T cell commitment. Consequently, our studies identify a source of extrathymic progenitors that will be helpful in defining the role of the thymus in the earliest steps of T cell differentiation.
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