Polarisome meets spitzenkörper: microscopy, genetics, and genomics converge - PubMed (original) (raw)

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Polarisome meets spitzenkörper: microscopy, genetics, and genomics converge

Steven D Harris et al. Eukaryot Cell. 2005 Feb.

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Figures

FIG. 1.

(a to d) Live-cell imaging of growing hyphal tips of N. crassa (a to c) and A. nidulans (d). Scale bars = 2.5 μm. (a) Digitally enhanced phase-contrast image of an unstained hypha showing Spk composed of a phase-dark cloud of secretory vesicles (arrow) and a phase-bright core (arrowhead) (M. Uchida and R. W. Roberson, unpublished data). (b) Confocal image of a hypha stained with FM4-64 showing pronounced staining of vesicles (arrow) within the Spk (P. C. Hickey and N. D. Read, unpublished data). (c) Confocal image colabeled with β-tubulin-GFP (green, black arrow) and FM4-64 (red, white arrow) showing microtubules extending into Spk. (d) Confocal image colabeled with SepA-GFP and FM4-64 (red, arrow) showing colocalization of SepA (yellow, arrowhead). In the central region of Spk is the region occupied by microvesicles (see panels e to g) (E. R. Kalkman and N. D. Read, unpublished data). (e to g) TEM of a hyphal tip of A. nidulans. A cluster of microvesicles within the Spk core (asterisks) surrounded by apical vesicles (white arrows), Woronin bodies (white arrowheads), and microtubules (black arrows) are indicated in these images. (e) Standard transmission electron micrograph (60-nm-thick section). (f) Electron tomographic section (∼3-nm _z_-axis resolution) of a dual-axis tomogram taken at 200 kV. (g) Model of panel f showing the 3D distribution of apical cytoplasm through an ∼170-nm-thick section (Uchida and Roberson, unpublished). Scale bar = 250 nm.

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