The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2 - PubMed (original) (raw)

Figure 4.

TOG association with subcellular components. Representative images of oligodendrocytes that were fixed, permeabilized, and immunostained for TOG (A, D, G, and J), CLIP 115 (B), hnRNP A2 (E), and hnRNP K (H) or injected with fluorescent UTP-labeled A2RE RNA (K). Green channel: A, D, G, and J and pairwise red channel: B, E, H, and K. TOG/CLIP (A–C), TOG/hnRNP A2 (D–F), TOG/hnRNP K (G–I), and TOG/A2RE RNA (J–L). Insets in B, E, H, and K: enlargements of a portion of cellular processes visualized as merged channels (A and B, D and E, G and H, and J and K, respectively). Yellow indicates spatial coincidence in the green and red channels. Bars, 20 μm (A and B), 15 μm (D and E), 12 μm (G and H), and 10 μm (J and K). Single granule ratiometric analysis (C, F, I, and L) was performed on cells treated as described above by integrating the intensities in the green channel (A, D, G, and J) and red channel (B, E, H, and K), respectively, for each granule. Only well resolved granules located in the medial and distal portions of the cellular processes were analyzed. Each dot represents the coordinates of one granule. Number (n) of granules analyzed: n = 3647 (C), n = 3217 (F), n = 1854 (I), and n = 1897 (L).