mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis - PubMed (original) (raw)
Affiliations
- PMID: 15704646
Free article
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis
Krystyna Boczoń et al. Folia Histochem Cytobiol. 2004.
Free article
Abstract
The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis.
Similar articles
- Effect of aminoguanidine and albendazole on inducible nitric oxide synthase (iNOS) activity in T. spiralis-infected mice muscles.
Zeromski J, Boczoń K, Wandurska-Nowak E, Mozer-Lisewska I. Zeromski J, et al. Folia Histochem Cytobiol. 2005;43(3):157-9. Folia Histochem Cytobiol. 2005. PMID: 16201316 - Inhibition of nitric oxide production by aminoguanidine influences the number of Trichinella spiralis parasites in infected "low responders" (C57BL/6) and "high responders" (BALB/c) mice.
Kołodziej-Sobocińska M, Dziemian E, Machnicka-Rowińska B. Kołodziej-Sobocińska M, et al. Parasitol Res. 2006 Jul;99(2):194-6. doi: 10.1007/s00436-006-0144-9. Epub 2006 Mar 16. Parasitol Res. 2006. PMID: 16541259 - [Influence of free radicals on Trichinella spiralis infection in mice].
Kołodziej-Sobocińska M. Kołodziej-Sobocińska M. Wiad Parazytol. 2006;52(3):239-41. Wiad Parazytol. 2006. PMID: 17432248 Polish. - Nitric oxide mediates intestinal pathology but not immune expulsion during Trichinella spiralis infection in mice.
Lawrence CE, Paterson JC, Wei XQ, Liew FY, Garside P, Kennedy MW. Lawrence CE, et al. J Immunol. 2000 Apr 15;164(8):4229-34. doi: 10.4049/jimmunol.164.8.4229. J Immunol. 2000. PMID: 10754319 - Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection.
Boonmars T, Wu Z, Nagano I, Takahashi Y. Boonmars T, et al. Parasitol Res. 2005 Aug;97(1):13-20. doi: 10.1007/s00436-005-1359-x. Epub 2005 Jun 10. Parasitol Res. 2005. PMID: 15948011
Cited by
- The impact of l-arginine supplementation on the enteral phase of experimental Trichinella spiralis infection in treated and untreated mice.
Fadl HO, Amin NM, Wanas H, El-Din SS, Ibrahim HA, Aboulhoda BE, Bocktor NZ. Fadl HO, et al. J Parasit Dis. 2020 Dec;44(4):737-747. doi: 10.1007/s12639-020-01245-1. Epub 2020 Jul 25. J Parasit Dis. 2020. PMID: 33184541 Free PMC article. - Nitric oxide in parasitic infections: a friend or foe?
Omar M, Abdelal HO. Omar M, et al. J Parasit Dis. 2022 Dec;46(4):1147-1163. doi: 10.1007/s12639-022-01518-x. Epub 2022 Jul 18. J Parasit Dis. 2022. PMID: 36457767 Free PMC article. Review. - Interplay between proinflammatory cytokines, miRNA, and tissue lesions in Anisakis-infected Sprague-Dawley rats.
Hrabar J, Trumbić Ž, Bočina I, Bušelić I, Vrbatović A, Mladineo I. Hrabar J, et al. PLoS Negl Trop Dis. 2019 May 15;13(5):e0007397. doi: 10.1371/journal.pntd.0007397. eCollection 2019 May. PLoS Negl Trop Dis. 2019. PMID: 31091271 Free PMC article. - Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis.
Yang QL, Shen JQ, Xue Y, Cheng XB, Jiang ZH, Yang YC, Chen YD, Zhou XN. Yang QL, et al. Korean J Parasitol. 2015 Dec;53(6):777-83. doi: 10.3347/kjp.2015.53.6.777. Epub 2015 Dec 31. Korean J Parasitol. 2015. PMID: 26797449 Free PMC article. - Effect of myrrh and thyme on Trichinella spiralis enteral and parenteral phases with inducible nitric oxide expression in mice.
Attia RA, Mahmoud AE, Farrag HM, Makboul R, Mohamed ME, Ibraheim Z. Attia RA, et al. Mem Inst Oswaldo Cruz. 2015 Dec;110(8):1035-41. doi: 10.1590/0074-02760150295. Epub 2015 Dec 11. Mem Inst Oswaldo Cruz. 2015. PMID: 26676322 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials