Interleukin-1 beta down-regulates the expression of metabotropic glutamate receptor 5 in cultured human astrocytes - PubMed (original) (raw)
Interleukin-1 beta down-regulates the expression of metabotropic glutamate receptor 5 in cultured human astrocytes
Eleonora Aronica et al. J Neuroimmunol. 2005 Mar.
Abstract
Expression of metabotropic glutamate receptor 5 (mGluR5) protein is known to be plastic and to depend critically on the astrocytes' microenvironment. In the present study we investigated whether interleukins, which are involved in the immune response following brain injury, could contribute to the regulation of mGluR5 protein in human astrocytes in culture. Using Western blotting and immunocytochemistry, no detectable changes in the expression of the mGluR5 protein were observed with both interleukin 1beta and interleukin 6 in undifferentiated cultures (growing in serum free media). In contrast, in cultures that had been morphologically differentiated by exposure to epidermal growth factor (EGF), addition of interleukin 1beta (but not interleukin 6) reduced mGluR5 protein expression. In addition, stimulation of phosphoinositide hydrolysis by the selective group I agonist (S)-3,5-dihydroxyphenylglycine (DHPG) was reduced after exposure to interleukin 1beta. The suppressive effect on mGluR5 was prevented by the interleukin 1 receptor antagonist. Thus, interleukin 1beta may represent an additional pathway through which mGluR5 expression and function can be modulated in astrocytes under different pathological conditions associated with an inflammatory response.
Similar articles
- Activation of metabotropic glutamate receptor 3 enhances interleukin (IL)-1beta-stimulated release of IL-6 in cultured human astrocytes.
Aronica E, Gorter JA, Rozemuller AJ, Yankaya B, Troost D. Aronica E, et al. Neuroscience. 2005;130(4):927-33. doi: 10.1016/j.neuroscience.2004.10.024. Neuroscience. 2005. PMID: 15652990 - Metabotropic glutamate receptor agonists potentiate cyclic AMP formation induced by forskolin or beta-adrenergic receptor activation in cerebral cortical astrocytes in culture.
Balázs R, Miller S, Chun Y, O'Toole J, Cotman CW. Balázs R, et al. J Neurochem. 1998 Jun;70(6):2446-58. doi: 10.1046/j.1471-4159.1998.70062446.x. J Neurochem. 1998. PMID: 9603209 - Metabotropic glutamate receptor mGluR5 in astrocytes: pharmacological properties and agonist regulation.
Balázs R, Miller S, Romano C, de Vries A, Chun Y, Cotman CW. Balázs R, et al. J Neurochem. 1997 Jul;69(1):151-63. doi: 10.1046/j.1471-4159.1997.69010151.x. J Neurochem. 1997. PMID: 9202306 - Exposure of astrocytes to thrombin reduces levels of the metabotropic glutamate receptor mGluR5.
Miller S, Sehati N, Romano C, Cotman CW. Miller S, et al. J Neurochem. 1996 Oct;67(4):1435-47. doi: 10.1046/j.1471-4159.1996.67041435.x. J Neurochem. 1996. PMID: 8858925
Cited by
- Therapeutic potential of mGluR5 targeting in Alzheimer's disease.
Kumar A, Dhull DK, Mishra PS. Kumar A, et al. Front Neurosci. 2015 Jun 9;9:215. doi: 10.3389/fnins.2015.00215. eCollection 2015. Front Neurosci. 2015. PMID: 26106290 Free PMC article. Review. - Increased matrix metalloproteinases expression in tuberous sclerosis complex: modulation by microRNA 146a and 147b in vitro.
Broekaart DWM, van Scheppingen J, Anink JJ, Wierts L, van Het Hof B, Jansen FE, Spliet WG, van Rijen PC, Kamphuis WW, de Vries HE, Aronica E, van Vliet EA. Broekaart DWM, et al. Neuropathol Appl Neurobiol. 2020 Feb;46(2):142-159. doi: 10.1111/nan.12572. Epub 2019 Jul 1. Neuropathol Appl Neurobiol. 2020. PMID: 31183875 Free PMC article. - microRNA-132 is overexpressed in glia in temporal lobe epilepsy and reduces the expression of pro-epileptogenic factors in human cultured astrocytes.
Korotkov A, Broekaart DWM, Banchaewa L, Pustjens B, van Scheppingen J, Anink JJ, Baayen JC, Idema S, Gorter JA, van Vliet EA, Aronica E. Korotkov A, et al. Glia. 2020 Jan;68(1):60-75. doi: 10.1002/glia.23700. Epub 2019 Aug 13. Glia. 2020. PMID: 31408236 Free PMC article. - Metabotropic glutamate receptors in glial cells.
D'Antoni S, Berretta A, Bonaccorso CM, Bruno V, Aronica E, Nicoletti F, Catania MV. D'Antoni S, et al. Neurochem Res. 2008 Dec;33(12):2436-43. doi: 10.1007/s11064-008-9694-9. Epub 2008 Apr 26. Neurochem Res. 2008. PMID: 18438710 Review. - Role of brain inflammation in epileptogenesis.
Choi J, Koh S. Choi J, et al. Yonsei Med J. 2008 Feb 29;49(1):1-18. doi: 10.3349/ymj.2008.49.1.1. Yonsei Med J. 2008. PMID: 18306464 Free PMC article. Review.