Dendrimer interactions with hydrophobic fluorescent probes and human serum albumin - PubMed (original) (raw)

Dendrimer interactions with hydrophobic fluorescent probes and human serum albumin

D Shcharbin et al. J Fluoresc. 2005 Jan.

Abstract

The interactions between three types of polyamidoamine dendrimers (with anionic, cationic, and neutral charge on a surface) and fluorescent dye 1-anilinonaphthalene-8-sulfonate (ANS) were studied. Double fluorimetric titration method was employed to estimate a binding constant and the number of binding centers. As fluorescent probes can serve as models of toxin molecules, dendrimers, and human serum albumin (HSA) abilities to bind ANS were compared. In the presence of HSA and dendrimers, ANS located both in HSA and in dendrimers, but the interactions between ANS and HSA were stronger.

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