Tat peptide-mediated cellular delivery: back to basics - PubMed (original) (raw)
Review
. 2005 Feb 28;57(4):559-77.
doi: 10.1016/j.addr.2004.12.001. Epub 2005 Jan 6.
Affiliations
- PMID: 15722164
- DOI: 10.1016/j.addr.2004.12.001
Review
Tat peptide-mediated cellular delivery: back to basics
Hilary Brooks et al. Adv Drug Deliv Rev. 2005.
Abstract
Peptides are emerging as attractive drug delivery tools. The HIV Tat-derived peptide is a small basic peptide that has been successfully shown to deliver a large variety of cargoes, from small particles to proteins, peptides and nucleic acids. The 'transduction domain' or region conveying the cell penetrating properties appears to be confined to a small (9 amino acids) stretch of basic amino acids, with the sequence RKKRRQRRR [S. Ruben, A. Perkins, R. Purcell, K. Joung, R. Sia, R. Burghoff, W.A. Haseltine, C.A. Rosen, Structural and functional characterization of human immunodeficiency virus tat protein, J. Virol. 63 (1989) 1-8; S. Fawell, J. Seery, Y. Daikh, C. Moore, L.L. Chen, B. Pepinsky, J. Barsoum, Tat-mediated delivery of heterologous proteins into cells, Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 664-668; E. Vives, P. Brodin, B. Lebleu, A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus, J. Biol. Chem. 272 (1997) 16010-16017; S. Futaki, T. Suzuki, W. Ohashi, T. Yagami, S. Tanaka, K. Ueda, Y. Sugiura, Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery, J. Biol. Chem. 276 (2001) 5836-5840.]. The mechanism by which the Tat peptide adheres to, and crosses, the plasma membrane of cells is currently a topic of heated discussion in the literature, with varied findings being reported. This review aims to bring together some of those findings. Peptide interactions at the cell surface, and possible mechanisms of entry, will be discussed together with the effects of modifying the basic sequence and attaching a cargo.
Similar articles
- A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus.
Vivès E, Brodin P, Lebleu B. Vivès E, et al. J Biol Chem. 1997 Jun 20;272(25):16010-7. doi: 10.1074/jbc.272.25.16010. J Biol Chem. 1997. PMID: 9188504 - Intracellular cargo delivery using tat peptide and derivatives.
Zhao M, Weissleder R. Zhao M, et al. Med Res Rev. 2004 Jan;24(1):1-12. doi: 10.1002/med.10056. Med Res Rev. 2004. PMID: 14595670 Review. - Possible existence of common internalization mechanisms among arginine-rich peptides.
Suzuki T, Futaki S, Niwa M, Tanaka S, Ueda K, Sugiura Y. Suzuki T, et al. J Biol Chem. 2002 Jan 25;277(4):2437-43. doi: 10.1074/jbc.M110017200. Epub 2001 Nov 15. J Biol Chem. 2002. PMID: 11711547 - Structural variety of membrane permeable peptides.
Futaki S, Goto S, Suzuki T, Nakase I, Sugiura Y. Futaki S, et al. Curr Protein Pept Sci. 2003 Apr;4(2):87-96. doi: 10.2174/1389203033487261. Curr Protein Pept Sci. 2003. PMID: 12678848 Review. - Antennapedia and HIV transactivator of transcription (TAT) "protein transduction domains" promote endocytosis of high molecular weight cargo upon binding to cell surface glycosaminoglycans.
Console S, Marty C, García-Echeverría C, Schwendener R, Ballmer-Hofer K. Console S, et al. J Biol Chem. 2003 Sep 12;278(37):35109-14. doi: 10.1074/jbc.M301726200. Epub 2003 Jun 30. J Biol Chem. 2003. PMID: 12837762
Cited by
- Study on characteristics of in vitro culture and intracellular transduction of exogenous proteins in fibroblast cell line of Liaoning cashmere goat.
Hu PF, Guan WJ, Li XC, Zhang WX, Li CL, Ma YH. Hu PF, et al. Mol Biol Rep. 2013 Jan;40(1):327-36. doi: 10.1007/s11033-012-2064-3. Epub 2012 Oct 14. Mol Biol Rep. 2013. PMID: 23065271 - Peptide-Based Strategies for Targeted Tumor Treatment and Imaging.
Ayo A, Laakkonen P. Ayo A, et al. Pharmaceutics. 2021 Apr 2;13(4):481. doi: 10.3390/pharmaceutics13040481. Pharmaceutics. 2021. PMID: 33918106 Free PMC article. Review. - A cell-penetrating peptide exerts therapeutic effects against ischemic stroke by mediating the lysosomal degradation of sirtuin 5.
Xia Q, Zhang X, Zhan G, Zheng L, Mao M, Zhao Y, Zhao Y, Li X. Xia Q, et al. MedComm (2020). 2023 Dec 13;4(6):e436. doi: 10.1002/mco2.436. eCollection 2023 Dec. MedComm (2020). 2023. PMID: 38093788 Free PMC article. - Multilayered films fabricated from an oligoarginine-conjugated protein promote efficient surface-mediated protein transduction.
Jewell CM, Fuchs SM, Flessner RM, Raines RT, Lynn DM. Jewell CM, et al. Biomacromolecules. 2007 Mar;8(3):857-63. doi: 10.1021/bm0609442. Epub 2007 Feb 2. Biomacromolecules. 2007. PMID: 17269822 Free PMC article. - Synthesis at the interface of chemistry and biology.
Wu X, Schultz PG. Wu X, et al. J Am Chem Soc. 2009 Sep 9;131(35):12497-515. doi: 10.1021/ja9026067. J Am Chem Soc. 2009. PMID: 19689159 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources