Prevalence and genetic characterization of caliciviruses among children hospitalized for acute gastroenteritis in the United States - PubMed (original) (raw)
Multicenter Study
Prevalence and genetic characterization of caliciviruses among children hospitalized for acute gastroenteritis in the United States
Carmelann Zintz et al. Infect Genet Evol. 2005 Apr.
Abstract
Human calicivirus was the first recognized viral agent causing gastroenteritis in humans. Norovirus (NV) and Sapovirus (SV), two genera within the Caliciviridae family, cause epidemic and endemic acute gastroenteritis in children and adults. The role of these viruses as a cause of sporadic acute gastroenteritis in young children requiring hospitalization is not well established. The aim of this study was to assess the prevalence and genetic diversity of caliciviruses among children hospitalized with symptoms of acute gastroenteritis. Stool samples were collected over 2 years from symptomatic children (N=1840) up to 5 years of age at three pediatric hospitals in the US. Overall, 156 (8.5%) samples were CV-positive, 131 (7.1%) confirmed by sequencing to be NV and 25 (1.4%) confirmed to be SV. Sequences of RT-PCR-amplified polymerase gene segments were analyzed using distance, maximum likelihood and parsimony algorithms. Phylogenetic analysis of 97 NV sequences showed that seven strains were in genogroup I, 86 strains were in genogroup II and four strains were not in genogroup I, II, or III, likely representing three new NV genogroups IV, VI and VII. Genogroup I and genogroup II strains were in 12 new genetic clusters, three in genogroup I and nine in genogroup II. Within genogroups I and II, most (98%) NV strains were in genetic clusters with no known prototype in GenBank. Phylogenetic analysis of 24 SV strains showed that half grouped with the London/92 strain in one genogroup and the remainder in three other proposed genogroups, one novel. In conclusion, NV and SV were frequent causes of hospitalization for acute gastroenteritis in young children and infecting strains were highly diverse, including newly recognized genogroups and genetic clusters within known genogroups.
Similar articles
- Genetic diversity of norovirus, sapovirus, and astrovirus isolated from children hospitalized with acute gastroenteritis in Chiang Mai, Thailand.
Malasao R, Maneekarn N, Khamrin P, Pantip C, Tonusin S, Ushijima H, Peerakome S. Malasao R, et al. J Med Virol. 2008 Oct;80(10):1749-55. doi: 10.1002/jmv.21244. J Med Virol. 2008. PMID: 18712816 - Detection and characterization of human caliciviruses in hospitalized children with acute gastroenteritis in Blantyre, Malawi.
Dove W, Cunliffe NA, Gondwe JS, Broadhead RL, Molyneux ME, Nakagomi O, Hart CA. Dove W, et al. J Med Virol. 2005 Dec;77(4):522-7. doi: 10.1002/jmv.20488. J Med Virol. 2005. PMID: 16254959 - Human sapoviruses: genetic diversity, recombination, and classification.
Hansman GS, Oka T, Katayama K, Takeda N. Hansman GS, et al. Rev Med Virol. 2007 Mar-Apr;17(2):133-41. doi: 10.1002/rmv.533. Rev Med Virol. 2007. PMID: 17340567 Review. - The epidemiology of human calicivirus/Sapporo/82/Japan.
Nakata S, Kogawa K, Numata K, Ukae S, Adachi N, Matson DO, Estes MK, Chiba S. Nakata S, et al. Arch Virol Suppl. 1996;12:263-70. doi: 10.1007/978-3-7091-6553-9_28. Arch Virol Suppl. 1996. PMID: 9015123 Review.
Cited by
- Herd immunity to GII.4 noroviruses is supported by outbreak patient sera.
Cannon JL, Lindesmith LC, Donaldson EF, Saxe L, Baric RS, Vinjé J. Cannon JL, et al. J Virol. 2009 Jun;83(11):5363-74. doi: 10.1128/JVI.02518-08. Epub 2009 Mar 18. J Virol. 2009. PMID: 19297483 Free PMC article. - Case-control study of diarrheal disease etiology in individuals over 5 years in southwest China.
Zhang SX, Yang CL, Gu WP, Ai L, Serrano E, Yang P, Zhou X, Li SZ, Lv S, Dang ZS, Chen JH, Hu W, Tian LG, Chen JX, Zhou XN. Zhang SX, et al. Gut Pathog. 2016 Nov 16;8:58. doi: 10.1186/s13099-016-0141-1. eCollection 2016. Gut Pathog. 2016. PMID: 27891182 Free PMC article. - Norovirus.
Robilotti E, Deresinski S, Pinsky BA. Robilotti E, et al. Clin Microbiol Rev. 2015 Jan;28(1):134-64. doi: 10.1128/CMR.00075-14. Clin Microbiol Rev. 2015. PMID: 25567225 Free PMC article. Review. - Identification of a porcine calicivirus related genetically to human sapoviruses.
Martella V, Lorusso E, Banyai K, Decaro N, Corrente M, Elia G, Cavalli A, Radogna A, Costantini V, Saif LJ, Lavazza A, Di Trani L, Buonavoglia C. Martella V, et al. J Clin Microbiol. 2008 Jun;46(6):1907-13. doi: 10.1128/JCM.00341-08. Epub 2008 Apr 16. J Clin Microbiol. 2008. PMID: 18417656 Free PMC article. - High prevalence of GII norovirus in hospitalized children with acute diarrhea, in Beijing.
Jia L, Zhang Y, Liu L, Dong H, Zhao L, Qian Y. Jia L, et al. PLoS One. 2017 Jun 29;12(6):e0179839. doi: 10.1371/journal.pone.0179839. eCollection 2017. PLoS One. 2017. PMID: 28662103 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical