SARS-associated coronavirus transmitted from human to pig - PubMed (original) (raw)

doi: 10.3201/eid1103.040824.

Minghua Yan, Ling Yang, Boliang Ding, Bo He, Yingzhen Wang, Xiuli Liu, Chenhui Liu, Hui Zhu, Bo You, Shengyong Huang, Jiangguo Zhang, Feng Mu, Zhao Xiang, Xiaoli Feng, Jie Wen, Jianqiu Fang, Jun Yu, Huanming Yang, Jian Wang

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SARS-associated coronavirus transmitted from human to pig

Weijun Chen et al. Emerg Infect Dis. 2005 Mar.

Abstract

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) was isolated from a pig during a survey for possible routes of viral transmission after a SARS epidemic. Sequence and epidemiology analyses suggested that the pig was infected by a SARS-CoV of human origin.

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Figures

Figure 1

Figure 1

Detection of antibodies against severe acute respiratory syndrome (SARS)–associated coronavirus recombinant proteins in animal sera by Western blotting. Recombinant nuleocapsid protein in panel A (NP, 54 kilodaltons [kDa]) and partial spike protein in panel B (SP, 57 kDa) were used as antigens. Goat anti-swine immunoglobulin G horseradish peroxidase was used as a secondary antibody. Serum samples from a convalescent SARS patient and healthy persons were used as positive and negative controls, respectively. Swine (S1 to S8) and human (H1 and H2) samples are sera collected during the survey. M1, M2, and M3 are purified NP, SP, and molecular weight markers, respectively. Positive bands at the corresponding molecular weight of the 2 proteins are indicated with arrows.

Figure 2

Figure 2

Phylogenetic analysis of severe acute respiratory syndrome–associated coronavirus S-gene. Nucleotide sequences of S genes (from 21491 to 25258 and 3768 bp in length) were compared. The result was displayed with MEGA-2 program and based on 125 complete S-gene sequences from GenBank.

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