Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites - PubMed (original) (raw)

. 2005 Mar 11;120(5):587-98.

doi: 10.1016/j.cell.2004.12.039.

Affiliations

Free article

Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites

Francene J Lemoine et al. Cell. 2005.

Free article

Abstract

In the yeast Saccharomyces cerevisiae, reduced levels of the replicative alpha DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. We selected translocations in a small region of chromosome III and found that they involve homologous recombination events between yeast retrotransposons (Ty elements) on chromosome III and retrotransposons located on other chromosomes. One of the two preferred sites of these translocations on chromosome III involve two Ty elements arrayed head-to-head; disruption of this site substantially reduces the rate of translocations. We demonstrate that this pair of Ty elements constitutes a preferred site for double-strand DNA breaks when DNA replication is compromised, analogous to the fragile sites observed in mammalian chromosomes.

PubMed Disclaimer

Comment in

Publication types

MeSH terms

Substances

LinkOut - more resources