In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag - PubMed (original) (raw)
In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag
Lawrence W Miller et al. Nat Methods. 2005 Apr.
Abstract
The introduction of green fluorescent protein and its variants (GFPs) has allowed protein analysis at the level of the cell. Now, chemical methods are needed to label proteins in vivo with a wider variety of functionalities so that mechanistic questions about protein function in the complex cellular environment can be addressed. Here we demonstrate that trimethoprim derivatives can be used to selectively tag Escherichia coli dihydrofolate reductase (eDHFR) fusion proteins in wild-type mammalian cells with minimal background and fast kinetics.
Similar articles
- Optimized fluorescent trimethoprim derivatives for in vivo protein labeling.
Calloway NT, Choob M, Sanz A, Sheetz MP, Miller LW, Cornish VW. Calloway NT, et al. Chembiochem. 2007 May 7;8(7):767-74. doi: 10.1002/cbic.200600414. Chembiochem. 2007. PMID: 17378009 - Chemical labeling strategies for cell biology.
Marks KM, Nolan GP. Marks KM, et al. Nat Methods. 2006 Aug;3(8):591-6. doi: 10.1038/nmeth906. Nat Methods. 2006. PMID: 16862131 Review. - A general method for the covalent labeling of fusion proteins with small molecules in vivo.
Keppler A, Gendreizig S, Gronemeyer T, Pick H, Vogel H, Johnsson K. Keppler A, et al. Nat Biotechnol. 2003 Jan;21(1):86-9. doi: 10.1038/nbt765. Epub 2002 Dec 9. Nat Biotechnol. 2003. PMID: 12469133 - Fluorescent labeling of proteins in living cells using the FKBP12 (F36V) tag.
Robers M, Pinson P, Leong L, Batchelor RH, Gee KR, Machleidt T. Robers M, et al. Cytometry A. 2009 Mar;75(3):207-24. doi: 10.1002/cyto.a.20649. Cytometry A. 2009. PMID: 18837033 - Selective chemical labeling of proteins in living cells.
Miller LW, Cornish VW. Miller LW, et al. Curr Opin Chem Biol. 2005 Feb;9(1):56-61. doi: 10.1016/j.cbpa.2004.12.007. Curr Opin Chem Biol. 2005. PMID: 15701454 Review.
Cited by
- Recent Progress in Regulating the Activity of Enzymes with Photoswitchable Inhibitors.
Chen Y. Chen Y. Molecules. 2024 Sep 24;29(19):4523. doi: 10.3390/molecules29194523. Molecules. 2024. PMID: 39407453 Free PMC article. Review. - The best of both worlds: Chemigenetic fluorescent sensors for biological imaging.
Tsao KK, Imai S, Chang M, Hario S, Terai T, Campbell RE. Tsao KK, et al. Cell Chem Biol. 2024 Sep 19;31(9):1652-1664. doi: 10.1016/j.chembiol.2024.08.002. Epub 2024 Sep 4. Cell Chem Biol. 2024. PMID: 39236713 Review. - Regulation of eDHFR-tagged proteins with trimethoprim PROTACs.
Etersque JM, Lee IK, Sharma N, Xu K, Ruff A, Northrup JD, Sarkar S, Nguyen T, Lauman R, Burslem GM, Sellmyer MA. Etersque JM, et al. Nat Commun. 2023 Nov 3;14(1):7071. doi: 10.1038/s41467-023-42820-3. Nat Commun. 2023. PMID: 37923771 Free PMC article. - Maximizing the performance of protein-based fluorescent biosensors.
Chai F, Cheng D, Nasu Y, Terai T, Campbell RE. Chai F, et al. Biochem Soc Trans. 2023 Aug 31;51(4):1585-1595. doi: 10.1042/BST20221413. Biochem Soc Trans. 2023. PMID: 37431791 Free PMC article. Review. - A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.
Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S. Baek I, et al. G3 (Bethesda). 2022 Sep 30;12(10):jkac201. doi: 10.1093/g3journal/jkac201. G3 (Bethesda). 2022. PMID: 35944214 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources