Association of polymorphisms of interleukin-18 gene promoter region with chronic hepatitis B in Chinese Han population - PubMed (original) (raw)
Association of polymorphisms of interleukin-18 gene promoter region with chronic hepatitis B in Chinese Han population
Ping-An Zhang et al. World J Gastroenterol. 2005.
Abstract
Aim: To investigate the polymorphisms of interleukin-18 (IL-18) gene promoters, and to disclose whether such polymorphisms are associated with susceptibility to chronic hepatitis B in Chinese Han population.
Methods: Using polymerase chain reaction with sequence specific primers (PCR-SSP) method, the single nucleotide polymorphisms (SNPs) of the promoter region of IL-18 gene at position -607 and -137 were detected in 231 patients with chronic hepatitis B and 300 normal controls.
Results: Allele C at position -607 in the promoter of IL-18 gene was detected in 48.7% of normal controls and 51.9% of patients, while allele A at position -607 was detected in 51.3% of normal controls and 48.1% of patients. The frequencies of -607CC, -607 CA and -607AA genotypes in normal controls were 22.0%, 53.3% and 24.7% respectively and in chronic hepatitis B patients were 26.8%, 50.2% and 23.0% respectively. Allele G at position -137 in the promoter of IL-18 gene was detected in 82.3% of normal controls and 88.5% of chronic hepatitis B patients, while allele C at position -137 was detected in 17.7% of normal controls and 11.5% of patients. The frequencies of -137GG, GC and CC genotype were 67.3%, 30.0% and 2.7% in normal controls respectively, while in chronic hepatitis B patients were 78.8%, 19.5% and 1.7% respectively. The frequency of -137GG genotype in chronic hepatitis B groups was significantly higher than that in normal controls (chi2 = 8.55, P = 0.003<0.05), whereas the frequencies of -607C/-137C and -607A/-137C haplotypes in chronic hepatitis B groups were significantly lower than that in normal controls. The association between genotypes of IL-18 promoter region polymorphisms and HBV copies showed that the frequency of -607AA genotype in high HBV-DNA copies groups was lower than that in low HBV-DNA copies groups (chi2 = 6.03, P = 0.014<0.05).
Conclusion: The polymorphisms of the promoter region of IL-18 gene at position -607 and -137 are closely associated with susceptibility to chronic hepatitis B. The people with allele C at position -137 in the promoter of IL-18 gene may be protected against HBV infection; moreover AA genotype at position -607 may be closely linked to inhibit HBV-DNA replication. These findings give some new clues to the study of pathogenesis of chronic hepatitis B.
Figures
Figure 1
Genotyping for the IL-18 position -137 and -607 polymorphisms. M: DL2000 DNA Marker; lanes 1–3: -137 GG, GC and CC; lanes 4-6: -607 CC, CA and AA.
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References
- Perrillo RP. How will we use the new antiviral agents for hepatitis B? Curr Gastroenterol Rep. 2002;4:63–71. - PubMed
- de Andrade DR, de Andrade DR. The influence of the human genome on chronic viral hepatitis outcome. Rev Inst Med Trop Sao Paulo. 2004;46:119–126. - PubMed
- Thio CL, Carrington M, Marti D, O'Brien SJ, Vlahov D, Nelson KE, Astemborski J, Thomas DL. Class II HLA alleles and hepatitis B virus persistence in African Americans. J Infect Dis. 1999;179:1004–1006. - PubMed
- Jiang YG, Wang YM. Association between HLA class II gene and severity of chronic hepatitis B. Shijie Huaren Xiaohua Zazhi. 2004;12:622–625.
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