Memory T cells have gene expression patterns intermediate between naive and effector - PubMed (original) (raw)
Memory T cells have gene expression patterns intermediate between naive and effector
Susan Holmes et al. Proc Natl Acad Sci U S A. 2005.
Abstract
The biological basis underlying differentiation of naive (NAI) T cells into effector (EFFE) and memory (MEM) cells is incompletely understood. Furthermore, whether NAI T cells serially differentiate into EFFE and then MEM cells (linear differentiation) or whether they concurrently differentiate into either EFFE or MEM cells (parallel differentiation) remains unresolved. We isolated NAI, EFFE, and MEM CD8(+) T cell subsets from human peripheral blood and analyzed their gene expression by using microarrays. We identified 156 genes that strongly differentiate NAI, EFFE, and MEM CD8(+) T cells; these genes provide previously unrecognized markers to help identify each cell type. Using several statistical approaches to analyze and group the data (standard heat-map and hierarchical clustering, a unique circular representation, multivariate analyses based on principal components, and a clustering method based on phylogenetic parsimony analysis), we assessed the lineage relationships between these subsets and showed that MEM cells have gene expression patterns intermediate between NAI and EFFE T cells. Our analysis suggests a common differentiation pathway to an intermediate state followed by a split into EFFE or MEM cells, hence supporting the parallel differentiation model. As such, conditions under which NAI T cells are activated may determine the magnitude of both EFFE and MEM cells, which arise subsequently. A better understanding of these conditions may be very useful in the design of future vaccine strategies to maximize MEM cell generation.
Figures
Fig. 1.
Boxplots of sample genes demonstrating expression ranges in each cell type: selectin-L (A), IL-7R (B), and granzyme. B (C).
Fig. 2.
Graphical representations of patterns of differential expression for the set of 156 significant genes: angular representation of genes (A), standardized scatter plot (B), and histogram of angles (C).
Fig. 3.
First principal plane from the principal components analysis of the matrix of significant genes in all 30 samples.
Fig. 4.
Tree representations of arrays: hierarchical clustering of arrays (A) and parsimony tree (B).
Similar articles
- Characterization of a CD44/CD122int memory CD8 T cell subset generated under sterile inflammatory conditions.
Mbitikon-Kobo FM, Vocanson M, Michallet MC, Tomkowiak M, Cottalorda A, Angelov GS, Coupet CA, Djebali S, Marçais A, Dubois B, Bonnefoy-Bérard N, Nicolas JF, Arpin C, Marvel J. Mbitikon-Kobo FM, et al. J Immunol. 2009 Mar 15;182(6):3846-54. doi: 10.4049/jimmunol.0802438. J Immunol. 2009. PMID: 19265164 - Negative regulation of NKG2D expression by IL-4 in memory CD8 T cells.
Ventre E, Brinza L, Schicklin S, Mafille J, Coupet CA, Marçais A, Djebali S, Jubin V, Walzer T, Marvel J. Ventre E, et al. J Immunol. 2012 Oct 1;189(7):3480-9. doi: 10.4049/jimmunol.1102954. Epub 2012 Aug 31. J Immunol. 2012. PMID: 22942430 - Microarray analysis reveals similarity between CD8+CD28- T cells from young and elderly persons, but not of CD8+CD28+ T cells.
Lazuardi L, Herndler-Brandstetter D, Brunner S, Laschober GT, Lepperdinger G, Grubeck-Loebenstein B. Lazuardi L, et al. Biogerontology. 2009 Apr;10(2):191-202. doi: 10.1007/s10522-008-9167-1. Epub 2008 Aug 27. Biogerontology. 2009. PMID: 18751903 - Transcription factor regulation of CD8+ T-cell memory and exhaustion.
Angelosanto JM, Wherry EJ. Angelosanto JM, et al. Immunol Rev. 2010 Jul;236:167-75. doi: 10.1111/j.1600-065X.2010.00927.x. Immunol Rev. 2010. PMID: 20636816 Review. - 'Normal counterparts' of nodal peripheral T-cell lymphoma.
Rüdiger T, Geissinger E, Müller-Hermelink HK. Rüdiger T, et al. Hematol Oncol. 2006 Dec;24(4):175-80. doi: 10.1002/hon.786. Hematol Oncol. 2006. PMID: 16783841 Review.
Cited by
- In-vitro model to mimic T cell subset change in human PDAC organoid co-culture.
Knoblauch M, Ma T, Beirith I, Koch D, Hofmann F, Heinrich K, Aghamaliev U, Sirtl S, Westphalen CB, Nieß H, Reichert M, Angele MK, Regel I, Bazhin AV, Werner J, Ilmer M, Renz BW. Knoblauch M, et al. J Cancer Res Clin Oncol. 2023 Nov;149(14):13051-13064. doi: 10.1007/s00432-023-05100-7. Epub 2023 Jul 20. J Cancer Res Clin Oncol. 2023. PMID: 37470855 Free PMC article. - Reframing How Physical Activity Reduces The Incidence of Clinically-Diagnosed Cancers: Appraising Exercise-Induced Immuno-Modulation As An Integral Mechanism.
Emery A, Moore S, Turner JE, Campbell JP. Emery A, et al. Front Oncol. 2022 Mar 14;12:788113. doi: 10.3389/fonc.2022.788113. eCollection 2022. Front Oncol. 2022. PMID: 35359426 Free PMC article. Review. - B Cells Control Mucosal-Associated Invariant T Cell Responses to Salmonella enterica Serovar Typhi Infection Through the CD85j HLA-G Receptor.
Salerno-Gonçalves R, Rezwan T, Luo D, Tettelin H, Sztein MB. Salerno-Gonçalves R, et al. Front Immunol. 2021 Oct 1;12:728685. doi: 10.3389/fimmu.2021.728685. eCollection 2021. Front Immunol. 2021. PMID: 34659215 Free PMC article. - Early Survival Prediction Framework in CD19-Specific CAR-T Cell Immunotherapy Using a Quantitative Systems Pharmacology Model.
Mueller-Schoell A, Puebla-Osorio N, Michelet R, Green MR, Künkele A, Huisinga W, Strati P, Chasen B, Neelapu SS, Yee C, Kloft C. Mueller-Schoell A, et al. Cancers (Basel). 2021 Jun 3;13(11):2782. doi: 10.3390/cancers13112782. Cancers (Basel). 2021. PMID: 34205020 Free PMC article. - Expanding CAR T cells in human platelet lysate renders T cells with in vivo longevity.
Torres Chavez A, McKenna MK, Canestrari E, Dann CT, Ramos CA, Lulla P, Leen AM, Vera JF, Watanabe N. Torres Chavez A, et al. J Immunother Cancer. 2019 Nov 28;7(1):330. doi: 10.1186/s40425-019-0804-9. J Immunother Cancer. 2019. PMID: 31779709 Free PMC article.
References
- Opferman, J. T., Ober, B. T. & Ashton-Rickardt, P. G. (1999) Science 283, 1745–1748. - PubMed
- Kaech, S., Tan, J., Wherry, E., Konieczny, B., Surh, C. & Ahmed, R. (2003) Nat. Immunol. 4, 1191–1198. - PubMed
- Kaech, S., Hemby, S., Kersh, E. & Ahmed, R. (2002) Cell 111, 837–851. - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials