Secreted semaphorins modulate synaptic transmission in the adult hippocampus - PubMed (original) (raw)

Sema3F can modulate synaptic transmission in rat hippocampal slices. A, B, D, E, Plots of effect of AP-Sema3F versus AP ligand (1-2 n

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) on the frequency (A, D) and amplitude (B, E) of mEPSCs recorded from adult wild-type rats. A, B, Data from DG granule cells; D, E, data from CA1 pyramidal neurons. Open bars indicate AP ligand data, and hatched bars indicate AP-Sema3F ligand data. The mEPSC frequency values for DG neurons are as follows: AP versus AP-Sema3F, at 1-2 n

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, 1.97 ± 0.19 Hz, n = 11 versus 4.53 ± 0.39 Hz, n = 15; p = 6.869 × 10-7. The mEPSC amplitude values recorded from DG neurons are as follows: AP versus AP-Sema3F, at 1-2 n

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, 7.49 ± 0.36 pA, n = 11 versus 10.18 ± 0.61 pA, n = 15; p = 0.0004. The mEPSC frequency values for CA1 neurons are as follows: AP versus AP-Sema3F, at 1-2 n

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, 4.61 ± 0.84 Hz, n = 18 versus 7.31 ± 0.61 Hz, n = 29; p = 0.01122. The mEPSC amplitude values recorded from CA1 neurons are as follows: AP versus AP-Sema3F, at 1-2 n

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, 10.35 ± 0.41 pA, n = 18 versus 11.96 ± 0.30 pA, n = 29; p = 0.0025. Asterisk indicates statistical significance using two-tailed t test (α = 0.05). C, F, Example mEPSC traces from one representative rat dentate granule cell and one CA1 pyramidal neuron in AP- or AP-Sema3F-treated rat hippocampal slices. Calibration: 100 ms, 20 pA. G, Dose-response plot showing effects of bath-applied AP-Sema3F on frequency and amplitude of mEPSCs recorded from granule cells in DG and pyramidal cells in CA1 region of 6- to 9-week-old rat hippocampus. Data are plotted as mean mEPSC frequency or amplitude as a percentage of control AP ligand. Open circles indicate the frequency of mEPSCs recorded from DG granule cells. The following values are the average ± SE; and p values were obtained using two-tailed t test (α = 0.05): AP versus AP-Sema3F, at 0.3 n

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, 2.79 ± 0.12 Hz, n = 18 versus 3.94 ± 0.23 Hz, n = 21, p = 4.816 × 10-5; at 1-2 n

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, 1.97 ± 0.19 Hz, n = 11 versus 4.53 ± 0.39 Hz, n = 15; p = 6.869 × 10-7. Filled circles indicate the amplitude of mEPSCs recorded from DG granule cells: AP versus AP-Sema3F, at 0.3 n

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, 7.83 ± 0.18 pA, n = 18 versus 8.31 ± 0.14 pA, n = 21, p = 0.0415; at 1-2 n

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, 7.49 ± 0.36 pA, n = 11 versus 10.18 ± 0.61 pA, n = 15, p = 0.0004). Open triangles indicate the frequency of mEPSCs recorded from CA1 pyramidal neurons: AP versus AP-Sema3F, at 0.3 n

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, 3.25 ± 0.44 Hz, n = 16 versus 3.01 ± 0.41 Hz, n = 20, p = 0.6919; at 1-2 n

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, 4.61 ± 0.84 Hz, n = 18 versus 7.31 ± 0.61 Hz, n = 29, p = 0.01122. Filled triangles indicate the amplitude of mEPSCs recorded from CA1 pyramidal neurons: AP versus AP-Sema3F, at 0.3 n

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, 9.95 ± 0.33 pA, n = 16 versus 9.88 ± 0.33 pA, n = 20, p = 0.8876; at 1-2 n

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, 10.35 ± 0.41 pA, n = 18 versus 11.96 ± 0.30 pA, n = 29, p = 0.0025). Asterisk indicates statistical significance using two-tailed t test (α = 0.05).