Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase - PubMed (original) (raw)
. 2005 Apr 20;127(15):5298-9.
doi: 10.1021/ja0509073.
Affiliations
- PMID: 15826149
- DOI: 10.1021/ja0509073
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase
Lee W Tremblay et al. J Am Chem Soc. 2005.
Abstract
This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
Similar articles
- Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis.
Zhang G, Dai J, Wang L, Dunaway-Mariano D, Tremblay LW, Allen KN. Zhang G, et al. Biochemistry. 2005 Jul 12;44(27):9404-16. doi: 10.1021/bi050558p. Biochemistry. 2005. PMID: 15996095 - The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction.
Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN. Lahiri SD, et al. Science. 2003 Mar 28;299(5615):2067-71. doi: 10.1126/science.1082710. Epub 2003 Mar 13. Science. 2003. PMID: 12637673 - Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis.
Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN. Lahiri SD, et al. Biochemistry. 2002 Jul 2;41(26):8351-9. doi: 10.1021/bi0202373. Biochemistry. 2002. PMID: 12081483 - Phosphoryl transfer enzymes and hypervalent phosphorus chemistry.
Holmes RR. Holmes RR. Acc Chem Res. 2004 Oct;37(10):746-53. doi: 10.1021/ar040053b. Acc Chem Res. 2004. PMID: 15491121 Review. - Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues.
Hrmova M, Fincher GB. Hrmova M, et al. Carbohydr Res. 2007 Sep 3;342(12-13):1613-23. doi: 10.1016/j.carres.2007.05.013. Epub 2007 May 18. Carbohydr Res. 2007. PMID: 17548065 Review.
Cited by
- An Enzyme with High Catalytic Proficiency Utilizes Distal Site Substrate Binding Energy to Stabilize the Closed State but at the Expense of Substrate Inhibition.
Robertson AJ, Cruz-Navarrete FA, Wood HP, Vekaria N, Hounslow AM, Bisson C, Cliff MJ, Baxter NJ, Waltho JP. Robertson AJ, et al. ACS Catal. 2022 Mar 4;12(5):3149-3164. doi: 10.1021/acscatal.1c05524. Epub 2022 Feb 22. ACS Catal. 2022. PMID: 35692864 Free PMC article. - Phosphoglucomutase Is Not the Target for Galactose Toxicity in Plants.
Althammer M, Blöchl C, Reischl R, Huber CG, Tenhaken R. Althammer M, et al. Front Plant Sci. 2020 Feb 28;11:167. doi: 10.3389/fpls.2020.00167. eCollection 2020. Front Plant Sci. 2020. PMID: 32180781 Free PMC article. - 1H, 15N and 13C backbone resonance assignments of the P146A variant of β-phosphoglucomutase from Lactococcus lactis in its substrate-free form.
Cruz-Navarrete FA, Baxter NJ, Wood HP, Hounslow AM, Waltho JP. Cruz-Navarrete FA, et al. Biomol NMR Assign. 2019 Oct;13(2):349-356. doi: 10.1007/s12104-019-09904-y. Epub 2019 Aug 8. Biomol NMR Assign. 2019. PMID: 31396843 Free PMC article. - Two symmetric arginine residues play distinct roles in Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.
Lei J, Sheng G, Cheung PP, Wang S, Li Y, Gao X, Zhang Y, Wang Y, Huang X. Lei J, et al. Proc Natl Acad Sci U S A. 2019 Jan 15;116(3):845-853. doi: 10.1073/pnas.1817041116. Epub 2018 Dec 27. Proc Natl Acad Sci U S A. 2019. PMID: 30591565 Free PMC article. - Crystal Structures of Type-II Inositol Polyphosphate 5-Phosphatase INPP5B with Synthetic Inositol Polyphosphate Surrogates Reveal New Mechanistic Insights for the Inositol 5-Phosphatase Family.
Mills SJ, Silvander C, Cozier G, Trésaugues L, Nordlund P, Potter BV. Mills SJ, et al. Biochemistry. 2016 Mar 8;55(9):1384-97. doi: 10.1021/acs.biochem.5b00838. Epub 2016 Feb 29. Biochemistry. 2016. PMID: 26854536 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources