Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides - PubMed (original) (raw)

Case Reports

. 1992 Mar;28A(3 Pt 1):161-7.

doi: 10.1007/BF02631086.

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Case Reports

Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides

K Kaltoft et al. In Vitro Cell Dev Biol. 1992 Mar.

Abstract

From a plaque biopsy of a patient with mycosis fungoides, two different continuous cell lines were established by including both IL-2 and IL-4 in the culture medium. Both continuous cell lines appeared with characteristic chromosome markers after approximately 40 cell population doublings. The initial karyotype recognized in T cells from the skin biopsy was 46,XY and the karyotypes of the continuous cell strains were 46,XY, -18, + i(18q) and another with multiple chromosome aberrations as described in Sezary T-cell leukemia. Phenotyping with monoclonal antibodies and T-cell receptor analysis indicates that the latter cell strain represents a minority of T-cells in the plaque. Due to its many chromosomal aberrations it probably represents the malignant cell, which may be a central cell in the immune stimulation taking place in the skin.

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