Wnt signaling inhibits adipogenesis through beta-catenin-dependent and -independent mechanisms - PubMed (original) (raw)
. 2005 Jun 24;280(25):24004-10.
doi: 10.1074/jbc.M501080200. Epub 2005 Apr 22.
Affiliations
- PMID: 15849360
- DOI: 10.1074/jbc.M501080200
Free article
Wnt signaling inhibits adipogenesis through beta-catenin-dependent and -independent mechanisms
Jennifer A Kennell et al. J Biol Chem. 2005.
Free article
Abstract
Wnt signaling has been reported to block apoptosis and regulate differentiation of mesenchymal progenitors through inhibition of glycogen synthase kinase 3 and stabilization of beta-catenin. The effects of Wnt in preadipocytes may be mediated through Frizzled (Fz) 1 and/or Fz2 as these Wnt receptors are expressed in preadipocytes and their expression declines upon induction of differentiation. We ectopically expressed constitutively active chimeras between Wnt8 and Fz1 or Fz2 in preadipocytes and mesenchymal precursor cells. Our results indicated that activated Fz1 increases stability of beta-catenin, inhibits apoptosis, induces osteoblastogenesis, and inhibits adipogenesis. Although activated Fz2 does not influence apoptosis or osteoblastogenesis, it inhibits adipogenesis through a mechanism independent of beta-catenin. An important mediator of the beta-catenin-independent pathway appears to be calcineurin because inhibitors of this serine/threonine phosphatase partially rescue the block to adipogenesis caused by Wnt3a or activated Fz2. These data supported a model in which Wnt signaling inhibits adipogenesis through both beta-catenin-dependent and beta-catenin-independent mechanisms.
Similar articles
- The human Frizzled 6 (HFz6) acts as a negative regulator of the canonical Wnt. beta-catenin signaling cascade.
Golan T, Yaniv A, Bafico A, Liu G, Gazit A. Golan T, et al. J Biol Chem. 2004 Apr 9;279(15):14879-88. doi: 10.1074/jbc.M306421200. Epub 2004 Jan 27. J Biol Chem. 2004. PMID: 14747478 - Wnt5b partially inhibits canonical Wnt/beta-catenin signaling pathway and promotes adipogenesis in 3T3-L1 preadipocytes.
Kanazawa A, Tsukada S, Kamiyama M, Yanagimoto T, Nakajima M, Maeda S. Kanazawa A, et al. Biochem Biophys Res Commun. 2005 May 6;330(2):505-10. doi: 10.1016/j.bbrc.2005.03.007. Biochem Biophys Res Commun. 2005. PMID: 15796911 - Wnt signaling protects 3T3-L1 preadipocytes from apoptosis through induction of insulin-like growth factors.
Longo KA, Kennell JA, Ochocinska MJ, Ross SE, Wright WS, MacDougald OA. Longo KA, et al. J Biol Chem. 2002 Oct 11;277(41):38239-44. doi: 10.1074/jbc.M206402200. Epub 2002 Aug 1. J Biol Chem. 2002. PMID: 12154096 - New steps in the Wnt/beta-catenin signal transduction pathway.
Sakanaka C, Sun TQ, Williams LT. Sakanaka C, et al. Recent Prog Horm Res. 2000;55:225-36. Recent Prog Horm Res. 2000. PMID: 11036939 Review. - Molecular switching of osteoblastogenesis versus adipogenesis: implications for targeted therapies.
Takada I, Kouzmenko AP, Kato S. Takada I, et al. Expert Opin Ther Targets. 2009 May;13(5):593-603. doi: 10.1517/14728220902915310. Expert Opin Ther Targets. 2009. PMID: 19397478 Review.
Cited by
- DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells.
Luo HH, Ren WY, Ye AH, Liu L, Jiang Y, Ye FL, He BC, Chen ZH. Luo HH, et al. Aging (Albany NY). 2024 Sep 26;16(18):12543-12558. doi: 10.18632/aging.206091. Epub 2024 Sep 26. Aging (Albany NY). 2024. PMID: 39331002 Free PMC article. - A Closer Look into White Adipose Tissue Biology and the Molecular Regulation of Stem Cell Commitment and Differentiation.
Dowker-Key PD, Jadi PK, Gill NB, Hubbard KN, Elshaarrawi A, Alfatlawy ND, Bettaieb A. Dowker-Key PD, et al. Genes (Basel). 2024 Aug 2;15(8):1017. doi: 10.3390/genes15081017. Genes (Basel). 2024. PMID: 39202377 Free PMC article. Review. - Unraveling Obesity: Transgenerational Inheritance, Treatment Side Effects, Flavonoids, Mechanisms, Microbiota, Redox Balance, and Bioavailability-A Narrative Review.
Naomi R, Teoh SH, Halim S, Embong H, Hasain Z, Bahari H, Kumar J. Naomi R, et al. Antioxidants (Basel). 2023 Aug 3;12(8):1549. doi: 10.3390/antiox12081549. Antioxidants (Basel). 2023. PMID: 37627544 Free PMC article. Review. - Targeting strategies for bone diseases: signaling pathways and clinical studies.
Xu H, Wang W, Liu X, Huang W, Zhu C, Xu Y, Yang H, Bai J, Geng D. Xu H, et al. Signal Transduct Target Ther. 2023 May 17;8(1):202. doi: 10.1038/s41392-023-01467-8. Signal Transduct Target Ther. 2023. PMID: 37198232 Free PMC article. Review. - The role of wnt signaling in diabetes-induced osteoporosis.
Bao K, Jiao Y, Xing L, Zhang F, Tian F. Bao K, et al. Diabetol Metab Syndr. 2023 Apr 28;15(1):84. doi: 10.1186/s13098-023-01067-0. Diabetol Metab Syndr. 2023. PMID: 37106471 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources