Niche-dependent translineage commitment of endothelial progenitor cells, not cell fusion in general, into myocardial lineage cells - PubMed (original) (raw)
Niche-dependent translineage commitment of endothelial progenitor cells, not cell fusion in general, into myocardial lineage cells
Satoshi Murasawa et al. Arterioscler Thromb Vasc Biol. 2005 Jul.
Abstract
Objective: Previous studies from our laboratory have shown therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial ischemia. Our purpose was to investigate the mechanisms regulating EPC contribution to myocardial regeneration.
Methods and results: To evaluate niche-dependent expression profiles of EPCs in vitro, we performed coculture using cultured EPCs derived from human peripheral blood and rat cardiac myoblast cell line (H9C2). Reverse-transcription polymerase chain reaction (PCR) disclosed the expression of human-specific cardiac markers as well as human-specific smooth muscle markers. Cytoimmunochemistry presented several cocultured cells stained with human specific cardiac antibody. To prove this translineage differentiation in vivo, human cultured EPCs were injected into nude rat myocardial infarction model. Reverse-transcription PCR as well as immunohistochemistry of rat myocardial samples demonstrated the expression of human specific cardiac, vascular smooth muscle, and endothelial markers. We observed the distribution of colors (Qtracker; Quantum Dot Corp) in coculture to detect the fused cells, and the frequency of cell fusion was <1%.
Conclusions: EPCs can contribute to not only vasculogenesis but also myogenesis in the ischemic myocardium in vivo. Transdifferentiation, not cell fusion, is dominant for EPCs commitment to myocardial lineage cells. Ex vivo expanded EPCs transplantation might have enhanced therapeutic potential for myocardial regeneration.
Similar articles
- Human umbilical cord-derived endothelial progenitor cells promote growth cytokines-mediated neorevascularization in rat myocardial infarction.
Hu CH, Li ZM, DU ZM, Zhang AX, Yang DY, Wu GF. Hu CH, et al. Chin Med J (Engl). 2009 Mar 5;122(5):548-55. Chin Med J (Engl). 2009. PMID: 19323906 - Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction.
Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T. Iwasaki H, et al. Circulation. 2006 Mar 14;113(10):1311-25. doi: 10.1161/CIRCULATIONAHA.105.541268. Circulation. 2006. PMID: 16534028 - Angiogenic murine endothelial progenitor cells are derived from a myeloid bone marrow fraction and can be identified by endothelial NO synthase expression.
Loomans CJ, Wan H, de Crom R, van Haperen R, de Boer HC, Leenen PJ, Drexhage HA, Rabelink TJ, van Zonneveld AJ, Staal FJ. Loomans CJ, et al. Arterioscler Thromb Vasc Biol. 2006 Aug;26(8):1760-7. doi: 10.1161/01.ATV.0000229243.49320.c9. Epub 2006 May 25. Arterioscler Thromb Vasc Biol. 2006. PMID: 16728651 - The relative potency and safety of endothelial progenitor cells and unselected mononuclear cells for recovery from myocardial infarction and ischemia.
Sekiguchi H, Ii M, Losordo DW. Sekiguchi H, et al. J Cell Physiol. 2009 May;219(2):235-42. doi: 10.1002/jcp.21672. J Cell Physiol. 2009. PMID: 19115244 Review. - Endogenous cardiac stem cells.
Barile L, Messina E, Giacomello A, Marbán E. Barile L, et al. Prog Cardiovasc Dis. 2007 Jul-Aug;50(1):31-48. doi: 10.1016/j.pcad.2007.03.005. Prog Cardiovasc Dis. 2007. PMID: 17631436 Review.
Cited by
- Endothelial-Mesenchymal Transition or Functional Tissue Regeneration - Two Outcomes of Heart Remodeling.
Šalingová B, Červenák Z, Adamičková A, Chomanicová N, Valášková S, Gažová A, Kyselovič J. Šalingová B, et al. Physiol Res. 2021 Nov 30;70(Suppl 1):S13-S20. doi: 10.33549/physiolres.934780. Physiol Res. 2021. PMID: 34918525 Free PMC article. Review. - Cyclic Stretch Induces Vascular Smooth Muscle Cells to Secrete Connective Tissue Growth Factor and Promote Endothelial Progenitor Cell Differentiation and Angiogenesis.
Yan J, Wang WB, Fan YJ, Bao H, Li N, Yao QP, Huo YL, Jiang ZL, Qi YX, Han Y. Yan J, et al. Front Cell Dev Biol. 2020 Dec 9;8:606989. doi: 10.3389/fcell.2020.606989. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33363166 Free PMC article. - Hyaluronan-Based Grafting Strategies for Liver Stem Cell Therapy and Tracking Methods.
Nevi L, Safarikia S, Di Matteo S, Biancaniello F, Chiappetta MF, Cardinale V. Nevi L, et al. Stem Cells Int. 2019 Jul 1;2019:3620546. doi: 10.1155/2019/3620546. eCollection 2019. Stem Cells Int. 2019. PMID: 31354838 Free PMC article. Review. - Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development.
Abd El Aziz MT, Abd El Nabi EA, Abd El Hamid M, Sabry D, Atta HM, Rahed LA, Shamaa A, Mahfouz S, Taha FM, Elrefaay S, Gharib DM, Elsetohy KA. Abd El Aziz MT, et al. J Adv Res. 2015 Mar;6(2):133-44. doi: 10.1016/j.jare.2013.12.006. Epub 2013 Dec 21. J Adv Res. 2015. PMID: 25750747 Free PMC article. - JAK-STAT signaling in cardiomyogenesis of cardiac stem cells.
Mohri T, Iwakura T, Nakayama H, Fujio Y. Mohri T, et al. JAKSTAT. 2012 Apr 1;1(2):125-30. doi: 10.4161/jkst.20296. JAKSTAT. 2012. PMID: 24058761 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical