Progesterone receptor isoforms A and B: temporal and spatial differences in expression during murine mammary gland development - PubMed (original) (raw)

. 2005 Aug;146(8):3577-88.

doi: 10.1210/en.2005-0346. Epub 2005 May 5.

Affiliations

• PMID: 15878961
• DOI: 10.1210/en.2005-0346

Progesterone receptor isoforms A and B: temporal and spatial differences in expression during murine mammary gland development

Mark D Aupperlee et al. Endocrinology. 2005 Aug.

Abstract

Progesterone is a potent mitogen in the mammary gland. Based on studies using cells and animals engineered to express progesterone receptor (PR) isoforms A or B, PRA and PRB are believed to have different functions. Using an immunohistochemical approach with antibodies specific for PRA only or PRB only, we show that PRA and PRB expression in mammary epithelial cells is temporally and spatially separated during normal mammary gland development in the BALB/c mouse. In the virgin mammary gland when ductal development is active, the only PR protein isoform expressed was PRA. PRA levels were significantly lower during pregnancy, suggesting a minor role at this stage of development. PRB was abundantly expressed only during pregnancy, during alveologenesis. PRA and PRB colocalization occurred in only a small percentage of cells. During pregnancy there was extensive colocalization of PRB with 5-bromo-2'-deoxyuridine (BrdU) and cyclin D1; 95% of BrdU-positive cells and 83% of cyclin D1-positive cells expressed PRB. No colocalization of PRA with either BrdU or cyclin D1 was observed at pregnancy. In the virgin gland, PRA colocalization with BrdU or cyclin D1 was low; only 27% of BrdU-positive cells and 4% of cyclin D1-positive cells expressed PRA. The implication of these findings is that different actions of progesterone are mediated in PRB positive vs. PRA-positive cells in vivo. The spatial and temporal separation of PR isoform expression in mouse mammary gland provides a unique opportunity to determine the specific functions of PRA vs. PRB in vivo.

PubMed Disclaimer

Similar articles

• Progesterone receptor isoforms and proliferation in the rat mammary gland during development.
  Kariagina A, Aupperlee MD, Haslam SZ. Kariagina A, et al. Endocrinology. 2007 Jun;148(6):2723-36. doi: 10.1210/en.2006-1493. Epub 2007 Mar 1. Endocrinology. 2007. PMID: 17332059
• Differential hormonal regulation and function of progesterone receptor isoforms in normal adult mouse mammary gland.
  Aupperlee MD, Haslam SZ. Aupperlee MD, et al. Endocrinology. 2007 May;148(5):2290-300. doi: 10.1210/en.2006-1721. Epub 2007 Feb 22. Endocrinology. 2007. PMID: 17317767
• Overlapping and distinct expression of progesterone receptors A and B in mouse uterus and mammary gland during the estrous cycle.
  Mote PA, Arnett-Mansfield RL, Gava N, deFazio A, Mulac-Jericevic B, Conneely OM, Clarke CL. Mote PA, et al. Endocrinology. 2006 Dec;147(12):5503-12. doi: 10.1210/en.2006-0040. Epub 2006 Sep 15. Endocrinology. 2006. PMID: 16980438
• Progesterone receptor isoform functions in normal breast development and breast cancer.
  Kariagina A, Aupperlee MD, Haslam SZ. Kariagina A, et al. Crit Rev Eukaryot Gene Expr. 2008;18(1):11-33. doi: 10.1615/critreveukargeneexpr.v18.i1.20. Crit Rev Eukaryot Gene Expr. 2008. PMID: 18197783 Free PMC article. Review.

Cited by

• Design, Synthesis, and Evaluation of _B_-(Trifluoromethyl)phenyl Phosphine-Borane Derivatives as Novel Progesterone Receptor Antagonists.
  Miyajima Y, Ochiai K, Fujii S. Miyajima Y, et al. Molecules. 2024 Apr 2;29(7):1587. doi: 10.3390/molecules29071587. Molecules. 2024. PMID: 38611867 Free PMC article.
• The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats.
  Gao Z, Shao D, Zhao C, Liu H, Zhao X, Wei Q, Ma B. Gao Z, et al. Int J Mol Sci. 2023 May 16;24(10):8841. doi: 10.3390/ijms24108841. Int J Mol Sci. 2023. PMID: 37240191 Free PMC article.
• Activation function 1 of progesterone receptor is required for mammary development and regulation of RANKL during pregnancy.
  Lee SH, Yap YHY, Lim CL, Woo ARE, Lin VCL. Lee SH, et al. Sci Rep. 2022 Jul 19;12(1):12286. doi: 10.1038/s41598-022-16289-x. Sci Rep. 2022. PMID: 35854046 Free PMC article.
• Estrogen receptor-α signaling in post-natal mammary development and breast cancers.
  Rusidzé M, Adlanmérini M, Chantalat E, Raymond-Letron I, Cayre S, Arnal JF, Deugnier MA, Lenfant F. Rusidzé M, et al. Cell Mol Life Sci. 2021 Aug;78(15):5681-5705. doi: 10.1007/s00018-021-03860-4. Epub 2021 Jun 22. Cell Mol Life Sci. 2021. PMID: 34156490 Free PMC article. Review.
• Alterations in Progesterone Receptor Isoform Balance in Normal and Neoplastic Breast Cells Modulates the Stem Cell Population.
  Recouvreux MS, Diaz Bessone MI, Taruselli A, Todaro L, Lago Huvelle MA, Sampayo RG, Bissell MJ, Simian M. Recouvreux MS, et al. Cells. 2020 Sep 11;9(9):2074. doi: 10.3390/cells9092074. Cells. 2020. PMID: 32932770 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • GlyGen glycoinformatics resource
  • Guide to Pharmacology

• Research Materials

  • NCI CPTC Antibody Characterization Program