Goblet cell degranulation in isolated canine tracheal epithelium: response to exogenous ATP, ADP, and adenosine - PubMed (original) (raw)

Goblet cell degranulation in isolated canine tracheal epithelium: response to exogenous ATP, ADP, and adenosine

C W Davis et al. Am J Physiol. 1992 May.

Abstract

Mucin secretion by goblet cells was determined by quantifying degranulation events (DE) in isolated, superficial epithelium from canine trachea. The epithelium was isolated and explanted to a novel transparent, permeable support, and the goblet cells were visualized by video microscopy. Baseline degranulation events were quantified at 0.05 DE/min. Luminal ATP (10(-4) M, n = 10) stimulated a biphasic secretory response; a burst, maximum rate = 87.9 +/- 25.3, was followed by a plateau, rate = 1.9 +/- 0.3 DE/min. Serosal ATP elicited a complex set of responses: 9 cells failed to respond, 13 exhibited a trivial response, and 31 responded vigorously but with highly variable patterns of degranulation. Nonhydrolyzable 5'-adenylylimidodiphosphate caused degranulation from both sides of the epithelium. Luminal ADP and adenosine were ineffective. Serosal ADP and adenosine elicited a range of responses that was similar in diversity and magnitude to the ATP response. Our conclusions were as follows: 1) goblet cells in the superficial epithelium of the airway can be studied at the single-cell level in explants; 2) nucleotides stimulate goblet cells to secrete mucin; and 3) the goblet cell expresses different nucleotide receptors on its apical and basolateral membranes.

PubMed Disclaimer

Cited by

Cloning and expression of a human P2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy.
Parr CE, Sullivan DM, Paradiso AM, Lazarowski ER, Burch LH, Olsen JC, Erb L, Weisman GA, Boucher RC, Turner JT. Parr CE, et al. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3275-9. doi: 10.1073/pnas.91.8.3275. Proc Natl Acad Sci U S A. 1994. PMID: 8159738 Free PMC article.
P2Y2-receptor-mediated activation of a contralateral, lanthanide-sensitive calcium entry pathway in the human airway epithelium.
Bahra P, Mesher J, Li S, Poll CT, Danahay H. Bahra P, et al. Br J Pharmacol. 2004 Sep;143(1):91-8. doi: 10.1038/sj.bjp.0705913. Epub 2004 Aug 2. Br J Pharmacol. 2004. PMID: 15289296 Free PMC article.
Mechanophysical stimulations of mucin secretion in cultures of nasal epithelial cells.
Davidovich NE, Kloog Y, Wolf M, Elad D. Davidovich NE, et al. Biophys J. 2011 Jun 22;100(12):2855-64. doi: 10.1016/j.bpj.2011.04.040. Biophys J. 2011. PMID: 21689518 Free PMC article.
P2u purinoceptor regulation of mucin secretion in SPOC1 cells, a goblet cell line from the airways.
Abdullah LH, Davis SW, Burch L, Yamauchi M, Randell SH, Nettesheim P, Davis CW. Abdullah LH, et al. Biochem J. 1996 Jun 15;316 ( Pt 3)(Pt 3):943-51. doi: 10.1042/bj3160943. Biochem J. 1996. PMID: 8670174 Free PMC article.
The lactoperoxidase system links anion transport to host defense in cystic fibrosis.
Conner GE, Wijkstrom-Frei C, Randell SH, Fernandez VE, Salathe M. Conner GE, et al. FEBS Lett. 2007 Jan 23;581(2):271-8. doi: 10.1016/j.febslet.2006.12.025. Epub 2006 Dec 19. FEBS Lett. 2007. PMID: 17204267 Free PMC article.

Goblet cell degranulation in isolated canine tracheal epithelium: response to exogenous ATP, ADP, and adenosine - PubMed (original) (raw)