Microbes on the human vaginal epithelium - PubMed (original) (raw)

Comparative Study

. 2005 May 31;102(22):7952-7.

doi: 10.1073/pnas.0503236102. Epub 2005 May 23.

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Comparative Study

Microbes on the human vaginal epithelium

Richard W Hyman et al. Proc Natl Acad Sci U S A. 2005.

Abstract

Using solely a gene-based procedure, PCR amplification of the 16S ribosomal RNA gene coupled with very deep sequencing of the amplified products, the microbes on 20 human vaginal epithelia of healthy women have been identified and quantitated. The Lactobacillus content on these 20 healthy vaginal epithelia was highly variable, ranging from 0% to 100%. For four subjects, Lactobacillus was (virtually) the only bacterium detected. However, that Lactobacillus was far from clonal and was a mixture of species and strains. Eight subjects presented complex mixtures of Lactobacillus and other microbes. The remaining eight subjects had no Lactobacillus. Instead, Bifidobacterium, Gardnerella, Prevotella, Pseudomonas, or Streptococcus predominated.

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Figures

Fig. 1.

Fig. 1.

Relationship tree of the Lactobacillus contigs for subject 02. Subject 02's Lactobacillus contigs of length at least 1 kb supported by at least four good quality reads were subjected to cluster analysis. The result is presented as a relationship tree with individual contigs identified by their closest GenBank match. There is no “evolutionary distance” scale in the figure, because the lines and connections do not imply quantitative distances. Only qualitative distance is implied.

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