Cell microarrays and RNA interference chip away at gene function - PubMed (original) (raw)

Review

. 2005 Jun:37 Suppl:S25-30.

doi: 10.1038/ng1560.

Affiliations

• PMID: 15920526
• DOI: 10.1038/ng1560

Review

Cell microarrays and RNA interference chip away at gene function

Douglas B Wheeler et al. Nat Genet. 2005 Jun.

Abstract

The recent development of cell microarrays offers the potential to accelerate high-throughput functional genetic studies. The widespread use of RNA interference (RNAi) has prompted several groups to fabricate RNAi cell microarrays that make possible discrete, in-parallel transfection with thousands of RNAi reagents on a microarray slide. Though still a budding technology, RNAi cell microarrays promise to increase the efficiency, economy and ease of genome-wide RNAi screens in metazoan cells.

PubMed Disclaimer

Similar articles

• RNAi microarray analysis in cultured mammalian cells.
  Mousses S, Caplen NJ, Cornelison R, Weaver D, Basik M, Hautaniemi S, Elkahloun AG, Lotufo RA, Choudary A, Dougherty ER, Suh E, Kallioniemi O. Mousses S, et al. Genome Res. 2003 Oct;13(10):2341-7. doi: 10.1101/gr.1478703. Genome Res. 2003. PMID: 14525932 Free PMC article.
• High-throughput gene silencing using cell arrays.
  Vanhecke D, Janitz M. Vanhecke D, et al. Oncogene. 2004 Nov 1;23(51):8353-8. doi: 10.1038/sj.onc.1208027. Oncogene. 2004. PMID: 15517016 Review.
• Drosophila genome-wide RNAi screens: are they delivering the promise?
  Mathey-Prevot B, Perrimon N. Mathey-Prevot B, et al. Cold Spring Harb Symp Quant Biol. 2006;71:141-8. doi: 10.1101/sqb.2006.71.027. Cold Spring Harb Symp Quant Biol. 2006. PMID: 17381290 Review.
• Oncology studies using siRNA libraries: the dawn of RNAi-based genomics.
  Sachse C, Echeverri CJ. Sachse C, et al. Oncogene. 2004 Nov 1;23(51):8384-91. doi: 10.1038/sj.onc.1208072. Oncogene. 2004. PMID: 15517020 Review.
• Cell microarrays in drug discovery.
  Castel D, Pitaval A, Debily MA, Gidrol X. Castel D, et al. Drug Discov Today. 2006 Jul;11(13-14):616-22. doi: 10.1016/j.drudis.2006.05.015. Drug Discov Today. 2006. PMID: 16793530 Review.

Cited by

• Overview of micro- and nano-technology tools for stem cell applications: micropatterned and microelectronic devices.
  Cagnin S, Cimetta E, Guiducci C, Martini P, Lanfranchi G. Cagnin S, et al. Sensors (Basel). 2012 Nov 19;12(11):15947-82. doi: 10.3390/s121115947. Sensors (Basel). 2012. PMID: 23202240 Free PMC article. Review.
• The potential of high-content high-throughput microscopy in drug discovery.
  Starkuviene V, Pepperkok R. Starkuviene V, et al. Br J Pharmacol. 2007 Sep;152(1):62-71. doi: 10.1038/sj.bjp.0707346. Epub 2007 Jul 2. Br J Pharmacol. 2007. PMID: 17603554 Free PMC article. Review.
• Nanostructured Lipid-based Films for Substrate Mediated Applications in Biotechnology.
  Kang M, Tuteja M, Centrone A, Topgaard D, Leal C. Kang M, et al. Adv Funct Mater. 2018;28:10.1002/adfm.201704356. doi: 10.1002/adfm.201704356. Adv Funct Mater. 2018. PMID: 31080383 Free PMC article.
• Parallel imaging microfluidic cytometer.
  Ehrlich DJ, McKenna BK, Evans JG, Belkina AC, Denis GV, Sherr DH, Cheung MC. Ehrlich DJ, et al. Methods Cell Biol. 2011;102:49-75. doi: 10.1016/B978-0-12-374912-3.00003-1. Methods Cell Biol. 2011. PMID: 21704835 Free PMC article. Review.
• Microarray technology in obstetrics and gynecology: a guide for clinicians.
  Ward K. Ward K. Am J Obstet Gynecol. 2006 Aug;195(2):364-72. doi: 10.1016/j.ajog.2005.12.014. Epub 2006 Apr 17. Am J Obstet Gynecol. 2006. PMID: 16615920 Free PMC article. Review.

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database