Cytochrome P450 2J2 promotes the neoplastic phenotype of carcinoma cells and is up-regulated in human tumors - PubMed (original) (raw)
Cytochrome P450 2J2 promotes the neoplastic phenotype of carcinoma cells and is up-regulated in human tumors
Jian-Gang Jiang et al. Cancer Res. 2005.
Abstract
Cytochrome P450 (CYP) arachidonic acid epoxygenase 2J2 converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids, which exert diverse biological activities in cardiovascular system and endothelial cells. However, it is unknown whether this enzyme highly expresses and plays any role in cancer. In this study, we found that very strong and selective CYP2J2 expression was detected in human carcinoma tissues in 101 of 130 patients (77%) as well as eight human carcinoma cell lines but undetectable in adjacent normal tissues and nontumoric human cell lines by Western, reverse transcription-PCR, and immunohistochemical staining. In addition, forced overexpression of CYP2J2, and CYP BM3F87V or addition of epoxyeicosatrienoic acids (EET) in cultured carcinoma cell lines in vitro markedly accelerated proliferation by analyses of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell accounts, and cell cycle analysis, and protected carcinoma cells from apoptosis induced by tumor necrosis factor alpha (TNF-alpha) in cultures. In contrast, antisense 2J2 transfection or addition of epoxygenase inhibitors 17-ODYA inhibited proliferation and accelerated cell apoptosis induced by TNF-alpha. Examination of signaling pathways on the effects of CYP2J2 and EETs revealed activation of mitogen-activated protein kinases and PI3 kinase-AKT systems and elevation of epithelial growth factor receptor phosphorylation level. These results strongly suggest that CYP epoxygenase 2J2 plays a previously unknown role in promotion of the neoplastic cellular phenotype and in the pathogenesis of a variety of human cancers.
Similar articles
- Effects of cytochrome P450 arachidonic acid epoxygenases on the proliferation of tumor cells.
Jiang JG, Shen GF, Chen C, Fu XN, Wang DW. Jiang JG, et al. Ai Zheng. 2009 Jan;28(1):14-9. Epub 2009 Jan 5. Ai Zheng. 2009. PMID: 19448409 - Expression of cytochrome P450 arachidonic acid epoxygenase 2J2 in human tumor tissues and cell lines.
Jiang JG, Fu XN, Chen CL, Wang DW. Jiang JG, et al. Ai Zheng. 2009 Feb;28(2):93-6. Epub 2009 Feb 23. Ai Zheng. 2009. PMID: 19550113 - Cytochrome p450 epoxygenase promotes human cancer metastasis.
Jiang JG, Ning YG, Chen C, Ma D, Liu ZJ, Yang S, Zhou J, Xiao X, Zhang XA, Edin ML, Card JW, Wang J, Zeldin DC, Wang DW. Jiang JG, et al. Cancer Res. 2007 Jul 15;67(14):6665-74. doi: 10.1158/0008-5472.CAN-06-3643. Cancer Res. 2007. PMID: 17638876 - Epoxyeicosatrienoic acids, cell signaling and angiogenesis.
Fleming I. Fleming I. Prostaglandins Other Lipid Mediat. 2007 Jan;82(1-4):60-7. doi: 10.1016/j.prostaglandins.2006.05.003. Epub 2006 Jul 11. Prostaglandins Other Lipid Mediat. 2007. PMID: 17164133 Review. - Cytochrome P450 epoxygenase CYP2J2 and the risk of coronary artery disease.
Spiecker M, Liao J. Spiecker M, et al. Trends Cardiovasc Med. 2006 Aug;16(6):204-8. doi: 10.1016/j.tcm.2006.04.001. Trends Cardiovasc Med. 2006. PMID: 16839864 Review.
Cited by
- Let-7b inhibits human cancer phenotype by targeting cytochrome P450 epoxygenase 2J2.
Chen F, Chen C, Yang S, Gong W, Wang Y, Cianflone K, Tang J, Wang DW. Chen F, et al. PLoS One. 2012;7(6):e39197. doi: 10.1371/journal.pone.0039197. Epub 2012 Jun 25. PLoS One. 2012. PMID: 22761738 Free PMC article. - Epoxides and soluble epoxide hydrolase in cardiovascular physiology.
Imig JD. Imig JD. Physiol Rev. 2012 Jan;92(1):101-30. doi: 10.1152/physrev.00021.2011. Physiol Rev. 2012. PMID: 22298653 Free PMC article. Review. - Cytochrome P450 epoxygenase CYP2J2 attenuates nephropathy in streptozotocin-induced diabetic mice.
Chen G, Wang P, Zhao G, Xu G, Gruzdev A, Zeldin DC, Wang DW. Chen G, et al. Prostaglandins Other Lipid Mediat. 2011 Nov;96(1-4):63-71. doi: 10.1016/j.prostaglandins.2011.06.009. Epub 2011 Jun 30. Prostaglandins Other Lipid Mediat. 2011. PMID: 21742052 Free PMC article. - Rosuvastatin attenuates the elevation in blood pressure induced by overexpression of human C-reactive protein.
Li X, Yang G, Zhao G, Wu B, Edin ML, Zeldin DC, Wang DW. Li X, et al. Hypertens Res. 2011 Jul;34(7):869-75. doi: 10.1038/hr.2011.44. Epub 2011 May 12. Hypertens Res. 2011. PMID: 21562509 Free PMC article. - Cyp2c44 regulates prostaglandin synthesis, lymphangiogenesis, and metastasis in a mouse model of breast cancer.
Kesavan R, Frömel T, Zukunft S, Laban H, Geyer A, Naeem Z, Heidler J, Wittig I, Elwakeel E, Brüne B, Weigert A, Popp R, Fleming I. Kesavan R, et al. Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):5923-5930. doi: 10.1073/pnas.1921381117. Epub 2020 Mar 2. Proc Natl Acad Sci U S A. 2020. PMID: 32123095 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources