Identification of plasmids by PCR-based replicon typing - PubMed (original) (raw)
Identification of plasmids by PCR-based replicon typing
Alessandra Carattoli et al. J Microbiol Methods. 2005 Dec.
Abstract
The epidemiological importance of tracing plasmids conferring drug resistance prompted us to develop a PCR method based on replicons (inc/rep PCR) of the major plasmid incompatibility groups among Enterobacteriaceae. Eighteen pairs of primers were designed to perform 5 multiplex- and 3 simplex-PCRs, recognizing FIA, FIB, FIC, HI1, HI2, I1-Igamma, L/M, N, P, W, T, A/C, K, B/O, X, Y, F, and FIIA. The specificity of the method was tested on a collection of 61 reference plasmids and on 20 Salmonella enterica strains of different serotypes isolated in Italy. Results indicated that the inc/rep PCR method demonstrates high specificity and sensitivity in detecting replicons on reference plasmids and also revealed the presence of recurrent and common plasmids in epidemiologically unrelated Salmonella isolates of different serotypes. These results suggest that the method is potentially applicable to a large number of strains to trace the diffusion of specific multi-drug resistance plasmids in different environments.
Similar articles
- Partition locus-based classification of selected plasmids in Klebsiella pneumoniae, Escherichia coli and Salmonella enterica spp.: an additional tool.
Bousquet A, Henquet S, Compain F, Genel N, Arlet G, Decré D. Bousquet A, et al. J Microbiol Methods. 2015 Mar;110:85-91. doi: 10.1016/j.mimet.2015.01.019. Epub 2015 Jan 24. J Microbiol Methods. 2015. PMID: 25623509 - Conjugative transferability of the A/C plasmids from Salmonella enterica isolates that possess or lack bla(CMY) in the A/C plasmid backbone.
Poole TL, Edrington TS, Brichta-Harhay DM, Carattoli A, Anderson RC, Nisbet DJ. Poole TL, et al. Foodborne Pathog Dis. 2009 Dec;6(10):1185-94. doi: 10.1089/fpd.2009.0316. Foodborne Pathog Dis. 2009. PMID: 19743926 - Plasmid profiling and incompatibility grouping of multidrug resistant Salmonella enterica serovar Typhi isolates in Nairobi, Kenya.
Mutai WC, Waiyaki PG, Kariuki S, Muigai AWT. Mutai WC, et al. BMC Res Notes. 2019 Jul 16;12(1):422. doi: 10.1186/s13104-019-4468-9. BMC Res Notes. 2019. PMID: 31311578 Free PMC article. - Plasmid replicon typing.
Johnson TJ, Nolan LK. Johnson TJ, et al. Methods Mol Biol. 2009;551:27-35. doi: 10.1007/978-1-60327-999-4_3. Methods Mol Biol. 2009. PMID: 19521864 - Plasmid Localization and Partition in Enterobacteriaceae.
Bouet JY, Funnell BE. Bouet JY, et al. EcoSal Plus. 2019 Jun;8(2):10.1128/ecosalplus.ESP-0003-2019. doi: 10.1128/ecosalplus.ESP-0003-2019. EcoSal Plus. 2019. PMID: 31187729 Free PMC article. Review.
Cited by
- First detection of blaIMI-2 gene in a clinical Escherichia coli strain.
Rojo-Bezares B, Martín C, López M, Torres C, Sáenz Y. Rojo-Bezares B, et al. Antimicrob Agents Chemother. 2012 Feb;56(2):1146-7. doi: 10.1128/AAC.05478-11. Epub 2011 Nov 21. Antimicrob Agents Chemother. 2012. PMID: 22106212 Free PMC article. No abstract available. - Genomic insights into epidemic plasmids carrying bla CTX-M and mcr-1 genes in Escherichia coli from Lebanese broiler production.
Mikhayel M, Praud K, Leclercq S, Karam Sarkis D, Doublet B. Mikhayel M, et al. JAC Antimicrob Resist. 2024 Sep 18;6(5):dlae149. doi: 10.1093/jacamr/dlae149. eCollection 2024 Oct. JAC Antimicrob Resist. 2024. PMID: 39296921 Free PMC article. - Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Enterobacter cloacae from a Czech patient previously hospitalized in Sri Lanka.
Papagiannitsis CC, Studentova V, Chudackova E, Bergerova T, Hrabak J, Radej J, Novak I. Papagiannitsis CC, et al. Folia Microbiol (Praha). 2013 Nov;58(6):547-9. doi: 10.1007/s12223-013-0247-5. Epub 2013 Apr 2. Folia Microbiol (Praha). 2013. PMID: 23546833 No abstract available. - F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and bla CTX-M-55/-14/-65 in Escherichia coli from chickens in China.
Yang X, Liu W, Liu Y, Wang J, Lv L, Chen X, He D, Yang T, Hou J, Tan Y, Xing L, Zeng Z, Liu JH. Yang X, et al. Front Microbiol. 2014 Dec 16;5:688. doi: 10.3389/fmicb.2014.00688. eCollection 2014. Front Microbiol. 2014. PMID: 25566207 Free PMC article. - Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan.
Kawahara R, Seto K, Taguchi M, Nakajima C, Kumeda Y, Suzuki Y. Kawahara R, et al. J Clin Microbiol. 2015 Sep;53(9):3035-8. doi: 10.1128/JCM.01263-15. Epub 2015 Jul 1. J Clin Microbiol. 2015. PMID: 26135870 Free PMC article.