The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model - PubMed (original) (raw)
The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model
D Oesterhelt et al. EMBO J. 1985 Sep.
Abstract
The quantum yield of the primary photoreaction of HR478 was determined as phi(green) = 0.34 +/- 0.02 and that of the photochemical reconversion of HRL410 into HR578 as phi(blue) = 0.01 +/- 0.004. From steady-state illumination and flash-photolysis experiments, a quantitative description of HRL410 formation and decay was made leading to a model of the photocycle in isolated, light-adapted HR. This model satisfies all known facts about HR including its photochronic behaviour.
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