Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis - PubMed (original) (raw)

Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis

Anthony G Tsolaki et al. J Clin Microbiol. 2005 Jul.

Abstract

Beijing/W strains of Mycobacterium tuberculosis are geographically widespread and hypervirulent. To enhance our understanding of their origin and evolution, we sought phylogenetically informative large sequence polymorphisms (LSPs) within the Beijing/W family. Comparative whole-genome hybridization of Beijing/W strains revealed 21 LSPs, 7 of which were previously unreported. We show that some of these LSPs are unique event polymorphisms that can be used to define and subdivide the Beijing/W family. One LSP (RD105) was seen in all Beijing/W strains and thus serves as a useful marker for the identification of this family of strains. Additional LSPs (RD142, RD150, and RD181) further divided this family into four monophyletic subgroups, demonstrating a deeper population structure than previously appreciated. All Beijing/W strains were also observed to have an intact pks15/1 gene that is involved in the biosynthesis of a phenolic glycolipid, a putative virulence factor. A simple PCR assay using these Beijing/W strain-defining deletions will facilitate molecular epidemiological studies and may assist in the identification of the molecular basis of phenotypes associated with this important lineage of M. tuberculosis.

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Figures

FIG. 1.

FIG. 1.

Genetic diversity in the Beijing/W family of M. tuberculosis. Markers were identified that define this lineage within M. tuberculosis and also divide it into distinct subgroups. LSPs present in H37Rv and absent from the interrogated Beijing/W isolates are shown in black. Each row represents an isolate, and each column is a region of difference. All strains had the 7-bp insertion at pks15/1 and had CTG/ACC codons at katG463/gyrA95. Superscript a: strains indicated by a number only were from Tsolaki et al. (33), strains indicated by “Beijing” plus a number were from D. Van Soolingen, and strains indicated by “TN” numbers were from B. N. Kreisworth.

FIG. 2.

FIG. 2.

Beijing/W family of M. tuberculosis is monophyletic. The phylogeny shows that RD105 defines the Beijing/W family and is further subdivided into four groups by RD181, RD150, and RD142. All of the Beijing/W strains contain an intact pks15/1 gene and may produce functional PGL.

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