Intra- and interpatient variability of the hsp65 and 16S-23S intergenic gene region in Mycobacterium abscessus strains from patients with cystic fibrosis - PubMed (original) (raw)

Intra- and interpatient variability of the hsp65 and 16S-23S intergenic gene region in Mycobacterium abscessus strains from patients with cystic fibrosis

Brigitte König et al. J Clin Microbiol. 2005 Jul.

Abstract

To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic sequence (ITS). We detected two different hsp65 genes and correspondingly two ITS sequevars belonging to M. abscessus type I and type II.

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Figures

FIG. 1.

PCR-RFLP of hsp65. The amplimers of the partially amplified hsp65 gene were digested with BstEII and HaeIII and separated by 3% agarose gel electrophoresis. M, molecular size marker (50-bp ladder); lanes 1, M. chelonae reference strain (DSM 43804); lanes 2, M. abscessus reference strain (DSM 44196); lanes 3, one representative isolate from patient 1 (M. abscessus type I); lanes 4, one representative isolate from patient 5 (M. abscessus type II).

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