Colonic microbiota signatures across five northern European countries - PubMed (original) (raw)

Colonic microbiota signatures across five northern European countries

Christophe Lay et al. Appl Environ Microbiol. 2005 Jul.

Abstract

The composition of the colonic microbiota of 91 northern Europeans was characterized by fluorescent in situ hybridization using 18 phylogenetic probes. On average 75% of the bacteria were identified, and large interindividual variations were observed. Clostridium coccoides and Clostridium leptum were the dominant groups (28.0% and 25.2%), followed by the Bacteroides (8.5%). According to principal component analysis, no significant grouping with respect to geographic origin, age, or gender was observed.

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Figures

FIG. 1.

FIG. 1.

Score plot of the PCA analysis with focus on groupings of samples with respect to geographic origin using principal components PC1 and PC2. The score plot shows the clustering of different samples, and samples that lie close to each other are similar with respect to the measured variables. Conversely, samples that lie diametrically opposite to each other will be very different from each other. Crosses, French; open squares, German; open circles, Danish; filled squares, English; filled triangles, Dutch.

References

    1. Amann, R. I., W. Ludwig, and K. H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169. - PMC - PubMed
    1. Franks, A. H., H. J. Harmsen, G. C. Raangs, G. J. Jansen, F. Schut, and G. W. Welling. 1998. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64:3336-3345. - PMC - PubMed
    1. Harmsen, H. J., G. C. Raangs, T. He, J. E. Degener, and G. W. Welling. 2002. Extensive set of 16S rRNA-based probes for detection of bacteria in human feces. Appl. Environ. Microbiol. 68:2982-2990. - PMC - PubMed
    1. Lay, C., M. Sutren, V. Rochet, K. Saunier, J. Doré, and L. Rigottier-Gois. Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota. Environ. Microbiol., in press. - PubMed
    1. Mangin, I., R. Bonnet, P. Seksik, L. Rigottier-Gois, M. Sutren, Y. Bouhnik, C. Neut, M. D. Collins, J.-F. Colombel, P. Marteau, and J. Dore. 2004. Molecular inventory of faecal microflora in patients with Crohn's disease. FEMS Microbiol. Ecol. 50:25-36. - PubMed

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