A spectrophotometric assay for nitrate in an excess of nitrite - PubMed (original) (raw)
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A spectrophotometric assay for nitrate in an excess of nitrite
Nataliya Beda et al. Nitric Oxide. 2005 Sep.
Abstract
Miranda et al. have developed a method for simultaneous evaluation of nitrate and nitrite concentrations using reduction of nitrate by vanadium(III) combined with detection by the acidic Griess reaction [K.M. Miranda, M.G. Espey, D.A. Wink, A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite, Nitric Oxide 5 (2001) 62-71]. The sensitivity of the nitrate assay decline if the mixture analyzed contains a large excess of nitrite relative to nitrate, for instance, in the case of oxidation products of nitric oxide (NO) in aerated solutions, or in sweat. By this reason nitrite should be removed before the nitrate assay, if [NO2-]>>[NO3-]. Here we lay out an improved method allowing the above limitation to be erased, using sulfamic acid for nitrite removal. We also describe some modifications that enhance the reproducibility of the assay.
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- A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite.
Miranda KM, Espey MG, Wink DA. Miranda KM, et al. Nitric Oxide. 2001 Feb;5(1):62-71. doi: 10.1006/niox.2000.0319. Nitric Oxide. 2001. PMID: 11178938
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