Comprehensive statistical study of 452 BRCA1 missense substitutions with classification of eight recurrent substitutions as neutral - PubMed (original) (raw)

Comprehensive statistical study of 452 BRCA1 missense substitutions with classification of eight recurrent substitutions as neutral

S V Tavtigian et al. J Med Genet. 2006 Apr.

Abstract

Background: Genetic testing for hereditary cancer syndromes contributes to the medical management of patients who may be at increased risk of one or more cancers. BRCA1 and BRCA2 testing for hereditary breast and ovarian cancer is one such widely used test. However, clinical testing methods with high sensitivity for deleterious mutations in these genes also detect many unclassified variants, primarily missense substitutions.

Methods: We developed an extension of the Grantham difference, called A-GVGD, to score missense substitutions against the range of variation present at their position in a multiple sequence alignment. Combining two methods, co-occurrence of unclassified variants with clearly deleterious mutations and A-GVGD, we analysed most of the missense substitutions observed in BRCA1.

Results: A-GVGD was able to resolve known neutral and deleterious missense substitutions into distinct sets. Additionally, eight previously unclassified BRCA1 missense substitutions observed in trans with one or more deleterious mutations, and within the cross-species range of variation observed at their position in the protein, are now classified as neutral.

Discussion: The methods combined here can classify as neutral about 50% of missense substitutions that have been observed with two or more clearly deleterious mutations. Furthermore, odds ratios estimated for sets of substitutions grouped by A-GVGD scores are consistent with the hypothesis that most unclassified substitutions that are within the cross-species range of variation at their position in BRCA1 are also neutral. For most of these, clinical reclassification will require integrated application of other methods such as pooled family histories, segregation analysis, or validated functional assay.

PubMed Disclaimer

Conflict of interest statement

Competing interests: AMD, TJ, TS, and AZ are employees of Myriad Genetics, Inc., or Myriad Genetic Laboratories, Inc. SVT and AT own stock in Myriad Genetics, Inc. Results from this work bear on Myriad Genetic Laboratories' commercial test for mutations in BRCA1 and BRCA2.

Figures

Similar articles

Cited by

References

    1. Miki Y, Swensen J, Shattuck‐Eidens D, Futreal P A, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett L M, Ding W, Bell R, Rosenthal J, Hussey C, Tran T, McClure M, Frye C, Hattier T, Phelps R, Haugen‐Strano A, Katcher H, Yakumo K, Gholami Z, Shaffer D, Stone S, Bayer S, Wray C, Bogden R, Dayananth P, Ward J, Tonin P, Narod S, Bristow P K, Norris F H, Helvering L, Morrison P, Rosteck P, Lai M, Barrett J C, Lewis C, Neuhausen S, Cannon‐Albright L, Goldgar D, Wiseman R, Kamb A, Skolnick M H. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 199426666–71. - PubMed
    1. Wooster R, Bignell G, Lancaster J, Swift S, Seal S, Mangion J, Collins N, Gregory S, Gumbs C, Micklem G. Identification of the breast cancer susceptibility gene BRCA2. Nature 1995378789–792. - PubMed
    1. Tavtigian S V, Simard J, Rommens J, Couch F, Shattuck‐Eidens D, Neuhausen S, Merajver S, Thorlacius S, Offit K, Stoppa‐Lyonnet D, Belanger C, Bell R, Berry S, Bogden R, Chen Q, Davis T, Dumont M, Frye C, Hattier T, Jammulapati S, Janecki T, Jiang P, Kehrer R, Leblanc J F, Mitchell J T, McArthur‐Morrison J, Nguyen K, Peng Y, Samson C, Schroeder M, Snyder S C, Steele L, Stringfellow M, Stroup C, Swedlund B, Swense J, Teng D, Thomas A, Tran T, Tranchant M, Weaver‐Feldhaus J, Wong A K, Shizuya H, Eyfjord J E, Cannon‐Albright L, Tranchant M, Labrie F, Skolnick M H, Weber B, Kamb A, Goldgar D E. The complete BRCA2 gene and mutations in chromosome 13q‐linked kindreds. Nat Genet 199612333–337. - PubMed
    1. Antoniou A, Pharoah P D, Narod S, Risch H A, Eyfjord J E, Hopper J L, Loman N, Olsson H, Johannsson O, Borg A, Pasini B, Radice P, Manoukian S, Eccles D M, Tang N, Olah E, Anton‐Culver H, Warner E, Lubinski J, Gronwald J, Gorski B, Tulinius H, Thorlacius S, Eerola H, Nevanlinna H, Syrjakoski K, Kallioniemi O P, Thompson D, Evans C, Peto J, Lalloo F, Evans D G, Easton D F. Average risks of breast and ovarian cancer associated with BRCA1 or BRCA2 mutations detected in case series unselected for family history: a combined analysis of 22 studies. Am J Hum Genet 2003721117–1130. - PMC - PubMed
    1. Thompson D, Easton D. The genetic epidemiology of breast cancer genes. J Mammary Gland Biol Neoplasia 20049221–236. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources