Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse - PubMed (original) (raw)
. 2005 Jul 14;436(7048):272-6.
doi: 10.1038/nature03681.
Affiliations
- PMID: 16015333
- DOI: 10.1038/nature03681
Free article
Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse
Lara S Collier et al. Nature. 2005.
Free article
Abstract
Retroviruses, acting as somatic cell insertional mutagens, have been widely used to identify cancer genes in the haematopoietic system and mammary gland. An insertional mutagen for use in other mouse somatic cells would facilitate the identification of genes involved in tumour formation in a wider variety of tissues. Here we report the ability of the Sleeping Beauty transposon to act as a somatic insertional mutagen to identify genes involved in solid tumour formation. A Sleeping Beauty transposon, engineered to elicit loss-of-function or gain-of-function mutations, transposed in all somatic tissues tested and accelerated tumour formation in mice predisposed to cancer. Cloning transposon insertion sites from these tumours revealed the presence of common integration sites, at known and candidate cancer genes, similar to those observed in retroviral mutagenesis screens. Sleeping Beauty is a new tool for unbiased, forward genetic screens for cancer genes in vivo.
Comment in
- Cancer biology: Sleeping Beauty awakens.
Weiser KC, Justice MJ. Weiser KC, et al. Nature. 2005 Jul 14;436(7048):184-6. doi: 10.1038/436184a. Nature. 2005. PMID: 16015313 No abstract available. - Transposons reanimated in mice.
Bestor TH. Bestor TH. Cell. 2005 Aug 12;122(3):322-5. doi: 10.1016/j.cell.2005.07.024. Cell. 2005. PMID: 16096053 Review.
Similar articles
- Cancer biology: Sleeping Beauty awakens.
Weiser KC, Justice MJ. Weiser KC, et al. Nature. 2005 Jul 14;436(7048):184-6. doi: 10.1038/436184a. Nature. 2005. PMID: 16015313 No abstract available. - Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system.
Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Dupuy AJ, et al. Nature. 2005 Jul 14;436(7048):221-6. doi: 10.1038/nature03691. Nature. 2005. PMID: 16015321 - Retroviral insertional mutagenesis: past, present and future.
Uren AG, Kool J, Berns A, van Lohuizen M. Uren AG, et al. Oncogene. 2005 Nov 21;24(52):7656-72. doi: 10.1038/sj.onc.1209043. Oncogene. 2005. PMID: 16299527 Review. - Hopping around the tumor genome: transposons for cancer gene discovery.
Collier LS, Largaespada DA. Collier LS, et al. Cancer Res. 2005 Nov 1;65(21):9607-10. doi: 10.1158/0008-5472.CAN-05-3085. Cancer Res. 2005. PMID: 16266976 Review. - Transposon vectors for gene-trap insertional mutagenesis in vertebrates.
Clark KJ, Geurts AM, Bell JB, Hackett PB. Clark KJ, et al. Genesis. 2004 Aug;39(4):225-33. doi: 10.1002/gene.20049. Genesis. 2004. PMID: 15286994
Cited by
- Embryonic Lethal Phenotyping to Identify Candidate Genes Related with Birth Defects.
Yan B, Gong B, Zheng Y, Sun L, Wu X. Yan B, et al. Int J Mol Sci. 2024 Aug 13;25(16):8788. doi: 10.3390/ijms25168788. Int J Mol Sci. 2024. PMID: 39201474 Free PMC article. - Preclinical Models of Hepatocellular Carcinoma: Current Utility, Limitations, and Challenges.
Cigliano A, Liao W, Deiana GA, Rizzo D, Chen X, Calvisi DF. Cigliano A, et al. Biomedicines. 2024 Jul 22;12(7):1624. doi: 10.3390/biomedicines12071624. Biomedicines. 2024. PMID: 39062197 Free PMC article. Review. - Advances in transposable elements: from mechanisms to applications in mammalian genomics.
Han M, Perkins MH, Novaes LS, Xu T, Chang H. Han M, et al. Front Genet. 2023 Nov 30;14:1290146. doi: 10.3389/fgene.2023.1290146. eCollection 2023. Front Genet. 2023. PMID: 38098473 Free PMC article. Review. - Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanoma.
Zhu EY, Schillo JL, Murray SD, Riordan JD, Dupuy AJ. Zhu EY, et al. iScience. 2023 Aug 31;26(10):107805. doi: 10.1016/j.isci.2023.107805. eCollection 2023 Oct 20. iScience. 2023. PMID: 37860756 Free PMC article. - Distinct shared and compartment-enriched oncogenic networks drive primary versus metastatic breast cancer.
Jiang Z, Ju Y, Ali A, Chung PED, Skowron P, Wang DY, Shrestha M, Li H, Liu JC, Vorobieva I, Ghanbari-Azarnier R, Mwewa E, Koritzinsky M, Ben-David Y, Woodgett JR, Perou CM, Dupuy A, Bader GD, Egan SE, Taylor MD, Zacksenhaus E. Jiang Z, et al. Nat Commun. 2023 Jul 18;14(1):4313. doi: 10.1038/s41467-023-39935-y. Nat Commun. 2023. PMID: 37463901 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials