The transmembrane glycoprotein of human immunodeficiency virus type 1 induces syncytium formation in the absence of the receptor binding glycoprotein - PubMed (original) (raw)

The transmembrane glycoprotein of human immunodeficiency virus type 1 induces syncytium formation in the absence of the receptor binding glycoprotein

L G Perez et al. J Virol. 1992 Jul.

Abstract

To study the intracellular transport and biological properties of the human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein (TM; gp41), we constructed a truncated envelope gene in which the majority of the coding sequences for the surface glycoprotein (SU; gp120) were deleted. Transient expression of this truncated env gene in primate cells resulted in the biosynthesis of two proteins with M(r)s of 52,000 and 41,000, respectively. Immunofluorescence studies with antibodies to the HIV-1 TM protein indicated that the intracellular and surface localization of these proteins were indistinguishable from those of the native HIV-1 gp120-gp41 complex. These results indicate that the oligosaccharide processing and cell surface transport of the HIV-1 TM protein were not dependent on the presence of the receptor binding subunit, gp120. Syncytium formation was readily detected upon expression of the deleted HIV-1 env gene into COS and CD4+ HeLa cell lines, suggesting that in the absence of gp120, the TM protein retained biological activity. This observation was confirmed by infection of primate and mouse cell lines with a recombinant vaccinia virus (vvgp41) expressing the truncated HIV-1 env gene. These results strongly suggest that (i) the two biological activities of the HIV-1 envelope glycoprotein can occur independently and (ii) the association of the two glycoprotein subunits may restrict the fusion activity of the transmembrane component to CD4+ cells.

PubMed Disclaimer

Cited by

Enhanced detection of respiratory pathogens with nanotrap particles.
Shafagati N, Fite K, Patanarut A, Baer A, Pinkham C, An S, Foote B, Lepene B, Kehn-Hall K. Shafagati N, et al. Virulence. 2016 Oct 2;7(7):756-69. doi: 10.1080/21505594.2016.1185585. Epub 2016 May 4. Virulence. 2016. PMID: 27145085 Free PMC article.
A proline-rich motif downstream of the receptor binding domain modulates conformation and fusogenicity of murine retroviral envelopes.
Lavillette D, Maurice M, Roche C, Russell SJ, Sitbon M, Cosset FL. Lavillette D, et al. J Virol. 1998 Dec;72(12):9955-65. doi: 10.1128/JVI.72.12.9955-9965.1998. J Virol. 1998. PMID: 9811733 Free PMC article.
The entire SU subunit is required for the incorporation of the HIV-1 envelope glycoprotein complex into virions.
Li YY, Perez LG. Li YY, et al. Virus Genes. 1997;14(3):211-23. doi: 10.1023/a:1007935927150. Virus Genes. 1997. PMID: 9311566
Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease.
Bukovsky AA, Dorfman T, Weimann A, Göttlinger HG. Bukovsky AA, et al. J Virol. 1997 Feb;71(2):1013-8. doi: 10.1128/JVI.71.2.1013-1018.1997. J Virol. 1997. PMID: 8995620 Free PMC article.
A conserved LXXLF sequence is the major determinant in p6gag required for the incorporation of human immunodeficiency virus type 1 Vpr.
Kondo E, Göttlinger HG. Kondo E, et al. J Virol. 1996 Jan;70(1):159-64. doi: 10.1128/JVI.70.1.159-164.1996. J Virol. 1996. PMID: 8523520 Free PMC article.

References

1. Cell. 1987 Sep 11;50(6):975-85 - PubMed
1. Cell. 1988 Apr 8;53(1):55-67 - PubMed
1. Adv Virus Res. 1989;36:107-51 - PubMed
1. Cell. 1989 Jun 30;57(7):1155-65 - PubMed
1. EMBO J. 1988 Feb;7(2):513-8 - PubMed

The transmembrane glycoprotein of human immunodeficiency virus type 1 induces syncytium formation in the absence of the receptor binding glycoprotein - PubMed (original) (raw)