Relationship between cerebral blood flow and oxygen metabolism, and extracellular glucose and lactate concentrations during middle cerebral artery occlusion and reperfusion: a microdialysis and positron emission tomography study in nonhuman primates - PubMed (original) (raw)
Relationship between cerebral blood flow and oxygen metabolism, and extracellular glucose and lactate concentrations during middle cerebral artery occlusion and reperfusion: a microdialysis and positron emission tomography study in nonhuman primates
Peter Frykholm et al. J Neurosurg. 2005 Jun.
Abstract
Object: Changes in lactate and glucose levels in the brain may be used to monitor a dynamic ischemic process. The authors related extracellular concentrations of glucose and lactate to regional cerebral blood flow (rCBF) and cerebral metabolic rate of oxygen (CMRO2) in a model of ischemia.
Methods: Transient (2 hours) middle cerebral artery occlusion (MCAO) was performed in eight macaque monkeys. Extracellular measurements of glucose and lactate levels using microdialysis (two probes in each brain) and sequential positron emission tomography measurements were performed during MCAO and reperfusion. Glucose and lactate levels were related to rCBF and CMRO2 as well as the pathophysiological categories of severe ischemia and penumbra. In probe regions characterized by severe ischemia, there were significant changes in glucose and lactate levels and the lactate/glucose ratio. In probe regions distinguished by penumbra, only lactate levels increased significantly and, in general, the changes were smaller and transient. This difference between severe ischemic and penumbral regions was significant for all microdialysis parameters. There was a significant correlation between glucose, and CBF and CMRO2. Lactate concentration was correlated with CMRO2.
Conclusions: Extracellular glucose levels might be limited by rCBF, whereas lactate levels were more related to CMRO2. Lactate concentration is a better marker of irreversible ischemia than glucose concentration, although near-zero levels of glucose during MCAO probably signals near-complete cessation of rCBF. In situations with elevated lactate levels, glucose may help to discriminate between partial and severe ischemia.
Similar articles
- Middle cerebral artery occlusion and reperfusion in primates monitored by microdialysis and sequential positron emission tomography.
Enblad P, Frykholm P, Valtysson J, Silander HC, Andersson J, Fasth KJ, Watanabe Y, Långström B, Hillered L, Persson L. Enblad P, et al. Stroke. 2001 Jul;32(7):1574-80. doi: 10.1161/01.str.32.7.1574. Stroke. 2001. PMID: 11441204 - A metabolic threshold of irreversible ischemia demonstrated by PET in a middle cerebral artery occlusion-reperfusion primate model.
Frykholm P, Andersson JL, Valtysson J, Silander HC, Hillered L, Persson L, Olsson Y, Yu WR, Westerberg G, Watanabe Y, Långström B, Enblad P. Frykholm P, et al. Acta Neurol Scand. 2000 Jul;102(1):18-26. doi: 10.1034/j.1600-0404.2000.102001018.x. Acta Neurol Scand. 2000. PMID: 10893058 - Increase of interstitial glycerol reflects the degree of ischaemic brain damage: a PET and microdialysis study in a middle cerebral artery occlusion-reperfusion primate model.
Frykholm P, Hillered L, Långström B, Persson L, Valtysson J, Watanabe Y, Enblad P. Frykholm P, et al. J Neurol Neurosurg Psychiatry. 2001 Oct;71(4):455-61. doi: 10.1136/jnnp.71.4.455. J Neurol Neurosurg Psychiatry. 2001. PMID: 11561027 Free PMC article. - Pathophysiology of the ischemic penumbra--revision of a concept.
Back T. Back T. Cell Mol Neurobiol. 1998 Dec;18(6):621-38. doi: 10.1023/a:1020629818207. Cell Mol Neurobiol. 1998. PMID: 9876870 Review. - Early postischemic hyperperfusion: pathophysiologic insights from positron emission tomography.
Marchal G, Young AR, Baron JC. Marchal G, et al. J Cereb Blood Flow Metab. 1999 May;19(5):467-82. doi: 10.1097/00004647-199905000-00001. J Cereb Blood Flow Metab. 1999. PMID: 10326714 Review.
Cited by
- Serotonergic neurons control cortical neuronal intracellular energy dynamics by modulating astrocyte-neuron lactate shuttle.
Natsubori A, Hirai S, Kwon S, Ono D, Deng F, Wan J, Miyazawa M, Kojima T, Okado H, Karashima A, Li Y, Tanaka KF, Honda M. Natsubori A, et al. iScience. 2023 Jan 5;26(1):105830. doi: 10.1016/j.isci.2022.105830. eCollection 2023 Jan 20. iScience. 2023. PMID: 36713262 Free PMC article. - Nutrition Therapy, Glucose Control, and Brain Metabolism in Traumatic Brain Injury: A Multimodal Monitoring Approach.
Kurtz P, Rocha EEM. Kurtz P, et al. Front Neurosci. 2020 Mar 24;14:190. doi: 10.3389/fnins.2020.00190. eCollection 2020. Front Neurosci. 2020. PMID: 32265626 Free PMC article. Review. - Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography.
Macaisa CM, Watabe T, Liu Y, Romanov V, Kanai Y, Horitsugi G, Kato H, Shimosegawa E, Hatazawa J. Macaisa CM, et al. Brain Sci. 2019 May 3;9(5):101. doi: 10.3390/brainsci9050101. Brain Sci. 2019. PMID: 31058865 Free PMC article. - Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia.
McDonough A, Lee RV, Noor S, Lee C, Le T, Iorga M, Phillips JLH, Murphy S, Möller T, Weinstein JR. McDonough A, et al. J Neurosci. 2017 Aug 23;37(34):8292-8308. doi: 10.1523/JNEUROSCI.0725-17.2017. Epub 2017 Jul 26. J Neurosci. 2017. PMID: 28747383 Free PMC article. - Let-7f Regulates the Hypoxic Response in Cerebral Ischemia by Targeting NDRG3.
Yao Y, Wang W, Jing L, Wang Y, Li M, Hou X, Wang J, Peng T, Teng J, Jia Y. Yao Y, et al. Neurochem Res. 2017 Feb;42(2):446-454. doi: 10.1007/s11064-016-2091-x. Epub 2016 Nov 4. Neurochem Res. 2017. PMID: 27812761
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources