Comparative sequence analysis of the Clostridium difficile toxins A and B - PubMed (original) (raw)
Comparative Study
. 1992 May;233(1-2):260-8.
doi: 10.1007/BF00587587.
Affiliations
- PMID: 1603068
- DOI: 10.1007/BF00587587
Comparative Study
Comparative sequence analysis of the Clostridium difficile toxins A and B
C von Eichel-Streiber et al. Mol Gen Genet. 1992 May.
Abstract
The six clones pTB112, pTB324, pTBs12, pCd122, pCd14 and pCd13 cover the tox locus of Clostridium difficile VPI 10463. This region of 19 kb of chromosomal DNA contains four open reading frames including the complete toxB and toxA genes. The two toxins show 63% amino acid (aa) homology, a relatedness that had been predicted by the cross-reactivity of some monoclonal antibodies (mAb) but that is in contrast to the toxin specificity of polyclonal antisera. A special feature of ToxA and ToxB is their repetitive C-termini. We define herein 19 individual CROPs (combined repetitive oligopeptides of 20-50 aa length) in the ToxB C-terminus, which are separable into five homologous groups. Comparison of the aa sequences of the N-terminal two-thirds of ToxA and ToxB revealed three marked structures, a cluster of 172 hydrophobic, highly conserved aa in the centre of both toxins, a sequence of 120 residues with an accumulation of highly conserved arginine, cysteine, histidine, methionine, and tryptophan residues, and a stretch of 248 less conserved aa. The probable function of these domains is discussed. Structural and functional homologies of ToxA and ToxB indicate that both genes have a common ancestor and may have evolved by gene duplication, with subsequent recombination and mutation, as has been reported for streptococcal glucosyltransferases (Gtf).
Similar articles
- Cloning of Clostridium difficile toxin B gene and demonstration of high N-terminal homology between toxin A and B.
von Eichel-Streiber C, Laufenberg-Feldmann R, Sartingen S, Schulze J, Sauerborn M. von Eichel-Streiber C, et al. Med Microbiol Immunol. 1990;179(5):271-9. doi: 10.1007/BF00192465. Med Microbiol Immunol. 1990. PMID: 2082160 - Clostridium difficile toxin A carries a C-terminal repetitive structure homologous to the carbohydrate binding region of streptococcal glycosyltransferases.
von Eichel-Streiber C, Sauerborn M. von Eichel-Streiber C, et al. Gene. 1990 Nov 30;96(1):107-13. doi: 10.1016/0378-1119(90)90348-u. Gene. 1990. PMID: 2148295 - Closing in on the toxic domain through analysis of a variant Clostridium difficile cytotoxin B.
von Eichel-Streiber C, Meyer zu Heringdorf D, Habermann E, Sartingen S. von Eichel-Streiber C, et al. Mol Microbiol. 1995 Jul;17(2):313-21. doi: 10.1111/j.1365-2958.1995.mmi_17020313.x. Mol Microbiol. 1995. PMID: 7494480 - An Update on Clostridium difficile Toxinotyping.
Rupnik M, Janezic S. Rupnik M, et al. J Clin Microbiol. 2016 Jan;54(1):13-8. doi: 10.1128/JCM.02083-15. Epub 2015 Oct 28. J Clin Microbiol. 2016. PMID: 26511734 Free PMC article. Review. - A family of clostridial and streptococcal ligand-binding proteins with conserved C-terminal repeat sequences.
Wren BW. Wren BW. Mol Microbiol. 1991 Apr;5(4):797-803. doi: 10.1111/j.1365-2958.1991.tb00752.x. Mol Microbiol. 1991. PMID: 1830357 Review.
Cited by
- Inhibition of Clostridioides difficile Toxins TcdA and TcdB by Ambroxol.
Heber S, Barthold L, Baier J, Papatheodorou P, Fois G, Frick M, Barth H, Fischer S. Heber S, et al. Front Pharmacol. 2022 Jan 4;12:809595. doi: 10.3389/fphar.2021.809595. eCollection 2021. Front Pharmacol. 2022. PMID: 35058787 Free PMC article. - Large Clostridial Toxins: Mechanisms and Roles in Disease.
Orrell KE, Melnyk RA. Orrell KE, et al. Microbiol Mol Biol Rev. 2021 Aug 18;85(3):e0006421. doi: 10.1128/MMBR.00064-21. Epub 2021 Jun 2. Microbiol Mol Biol Rev. 2021. PMID: 34076506 Free PMC article. Review. - Phylogenomics of 8,839 Clostridioides difficile genomes reveals recombination-driven evolution and diversification of toxin A and B.
Mansfield MJ, Tremblay BJ, Zeng J, Wei X, Hodgins H, Worley J, Bry L, Dong M, Doxey AC. Mansfield MJ, et al. PLoS Pathog. 2020 Dec 28;16(12):e1009181. doi: 10.1371/journal.ppat.1009181. eCollection 2020 Dec. PLoS Pathog. 2020. PMID: 33370413 Free PMC article. - Human α-Defensin-5 Efficiently Neutralizes Clostridioides difficile Toxins TcdA, TcdB, and CDT.
Korbmacher M, Fischer S, Landenberger M, Papatheodorou P, Aktories K, Barth H. Korbmacher M, et al. Front Pharmacol. 2020 Aug 12;11:1204. doi: 10.3389/fphar.2020.01204. eCollection 2020. Front Pharmacol. 2020. PMID: 32903430 Free PMC article. - Protective Effect of Pediococcus pentosaceus LI05 Against Clostridium difficile Infection in a Mouse Model.
Xu Q, Gu S, Chen Y, Quan J, Lv L, Chen D, Zheng B, Xu L, Li L. Xu Q, et al. Front Microbiol. 2018 Oct 9;9:2396. doi: 10.3389/fmicb.2018.02396. eCollection 2018. Front Microbiol. 2018. PMID: 30356740 Free PMC article.
References
- Microb Pathog. 1987 May;2(5):307-18 - PubMed
- Gene. 1990 Nov 30;96(1):107-13 - PubMed
- Infect Immun. 1982 Mar;35(3):1032-40 - PubMed
- Gene. 1988 Oct 30;70(2):343-50 - PubMed
- Rev Infect Dis. 1984 Mar-Apr;6 Suppl 1:S11-20 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources