Modulator effects of interleukin-1beta and tumor necrosis factor-alpha on AMPA-induced excitotoxicity in mouse organotypic hippocampal slice cultures - PubMed (original) (raw)

Morphological changes of Mac-1-positive microglial cells from resting to activated state in mouse hippocampal slice cultures exposed to AMPA. A-D, Localization of Mac-1/BrdU double staining (A, C) and PI uptake (B, D) in control culture (A, B) and culture exposed to 8 μ

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AMPA for 24 h, followed by 4 d of recovery in normal medium (C, D). The control cultures (A, B) display a random distribution of resting microglia, sporadic BrdU-positive cells (A), and low-basal PI uptake (B). Cultures exposed to 8 μ

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AMPA (C, D) display increased numbers of microglia and BrdU-positive cells in the lesion areas (CA1 and CA3; C) and a concomitant increase of PI uptake in the same subfields (D). E, Resting microglia in a control slice culture showing sporadic BrdU-positive cells. G, High-power photomicrograph of microglia stained for anti-Mac-1 located in CA1 pyramidal cell layer. Reactive microglia and an increase in the number of anti-BrdU-positive cells following 8 μ

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AMPA are shown. F, a, Double-labeled Mac-1 and BrdU-positive cell; b, activated Mac-1-positive cell; c, resting-like Mac-1-positive cell. H, High-power photomicrograph of labeled anti-BrdU-positive microglial cell located in CA1 pyramidal cell layer.