BBC3 mediates fenretinide-induced cell death in neuroblastoma - PubMed (original) (raw)

Expression of apoptotic genes induced by 4-HPR in NB cells. (a) Hierarchical clustering of apoptosis-related genes with ⩾2-fold induction of mRNA in the time course experiments in the 4-HPR sensitive SMS-KCNR cell line. Cells were treated with 5 μ

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4-HPR for 2, 4, 6, 8, 12, 24, 48 and 96 h. The heat map represents gene expression ratios: red indicates upregulation, green represents downregulation, and black indicates no change. The ratio color scale indicates the log2 ratios. Low-quality cDNA spots (spot quality <0.5, see Materials and methods) were marked with gray. (b) Fenretinide-induced transient upregulation of BBC3 in SH-SY5Y cells. In a time course experiment, protein samples were extracted from SH-SY5Y cells at 0, 12, 24, 30, 36 and 48 h after exposure to 5 μ

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4-HPR. An amount of 40 μg of protein of each sample was separated on 4–12% Bis–Tris gel and transferred to PVDF membrane. Antibodies against BBC3, BAK1 and BAX were used for Western blotting analysis. An anti-α-tubulin antibody was used for loading control on each Western blot. ImageQuant™ Image Analysis Software v.5.2 was used to quantify protein bands and the relative signal intensities (comparing to time 0) were shown in the plot after correcting for loading. ♦ represents BBC3, ▪ BAK1, and ▴ BAX