Role of Rho-kinase in regulation of insulin action and glucose homeostasis - PubMed (original) (raw)
doi: 10.1016/j.cmet.2005.06.011.
Pat Ongusaha, Wan Jin Jahng, Kazushi Araki, Cheol Soo Choi, Hyo-Jeong Kim, Yong Hee Lee, Kozo Kaibuchi, Barbara B Kahn, Hiroaki Masuzaki, Jason K Kim, Sam W Lee, Young-Bum Kim
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- PMID: 16098829
- DOI: 10.1016/j.cmet.2005.06.011
Free article
Role of Rho-kinase in regulation of insulin action and glucose homeostasis
Noboru Furukawa et al. Cell Metab. 2005 Aug.
Free article
Abstract
Accumulating evidence indicates an important role for serine phosphorylation of IRS-1 in the regulation of insulin action. Recent studies suggest that Rho-kinase (ROK) is a mediator of insulin signaling, via interaction with IRS-1. Here we show that insulin stimulation of glucose transport is impaired when ROK is chemically or biologically inhibited in cultured adipocytes and myotubes and in isolated soleus muscle ex vivo. Inactivation of ROK also reduces insulin-stimulated IRS-1 tyrosine phosphorylation and PI3K activity. Moreover, inhibition of ROK activity in mice causes insulin resistance by reducing insulin-stimulated glucose uptake in skeletal muscle in vivo. Mass spectrometry analysis identifies IRS-1 Ser632/635 as substrates of ROK in vitro, and mutation of these sites inhibits insulin signaling. These results strongly suggest that ROK regulates insulin-stimulated glucose transport in vitro and in vivo. Thus, ROK is an important regulator of insulin signaling and glucose metabolism.
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- R01 CA85681/CA/NCI NIH HHS/United States
- CA107574/CA/NCI NIH HHS/United States
- R01 DK043051/DK/NIDDK NIH HHS/United States
- R01-2005-000-10386/PHS HHS/United States
- R01 DK080756/DK/NIDDK NIH HHS/United States
- R01 DK43051/DK/NIDDK NIH HHS/United States
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