RNA interference is an antiviral defence mechanism in Caenorhabditis elegans - PubMed (original) (raw)

. 2005 Aug 18;436(7053):1044-7.

doi: 10.1038/nature03957.

Affiliations

• PMID: 16107852
• DOI: 10.1038/nature03957

RNA interference is an antiviral defence mechanism in Caenorhabditis elegans

Courtney Wilkins et al. Nature. 2005.

Abstract

RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-transcriptional gene silencing mechanism that is well defined genetically in Caenorhabditis elegans. RNAi has been postulated to function as an adaptive antiviral immune mechanism in the worm, but there is no experimental evidence for this. Part of the limitation is that there are no known natural viral pathogens of C. elegans. Here we describe an infection model in C. elegans using the mammalian pathogen vesicular stomatitis virus (VSV) to study the role of RNAi in antiviral immunity. VSV infection is potentiated in cells derived from RNAi-defective worm mutants (rde-1; rde-4), leading to the production of infectious progeny virus, and is inhibited in mutants with an enhanced RNAi response (rrf-3; eri-1). Because the RNAi response occurs in the absence of exogenously added VSV small interfering RNAs, these results show that RNAi is activated during VSV infection and that RNAi is a genuine antiviral immune defence mechanism in the worm.

PubMed Disclaimer

Similar articles

• Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegans.
  Lu R, Maduro M, Li F, Li HW, Broitman-Maduro G, Li WX, Ding SW. Lu R, et al. Nature. 2005 Aug 18;436(7053):1040-1043. doi: 10.1038/nature03870. Nature. 2005. PMID: 16107851 Free PMC article.
• Caenorhabditis elegans inositol hexaphosphate pathways couple to RNA interference and pathogen defense.
  Xu W, Sun Y, Breen P, Ruvkun G, Mao K. Xu W, et al. Proc Natl Acad Sci U S A. 2024 Dec 3;121(49):e2416982121. doi: 10.1073/pnas.2416982121. Epub 2024 Nov 27. Proc Natl Acad Sci U S A. 2024. PMID: 39602251 Free PMC article.
• TRIM69 Inhibits Vesicular Stomatitis Indiana Virus.
  Rihn SJ, Aziz MA, Stewart DG, Hughes J, Turnbull ML, Varela M, Sugrue E, Herd CS, Stanifer M, Sinkins SP, Palmarini M, Wilson SJ. Rihn SJ, et al. J Virol. 2019 Sep 30;93(20):e00951-19. doi: 10.1128/JVI.00951-19. Print 2019 Oct 15. J Virol. 2019. PMID: 31375575 Free PMC article.
• Depressing time: Waiting, melancholia, and the psychoanalytic practice of care.
  Salisbury L, Baraitser L. Salisbury L, et al. In: Kirtsoglou E, Simpson B, editors. The Time of Anthropology: Studies of Contemporary Chronopolitics. Abingdon: Routledge; 2020. Chapter 5. In: Kirtsoglou E, Simpson B, editors. The Time of Anthropology: Studies of Contemporary Chronopolitics. Abingdon: Routledge; 2020. Chapter 5. PMID: 36137063 Free Books & Documents. Review.
• Conservative, physical and surgical interventions for managing faecal incontinence and constipation in adults with central neurological diseases.
  Todd CL, Johnson EE, Stewart F, Wallace SA, Bryant A, Woodward S, Norton C. Todd CL, et al. Cochrane Database Syst Rev. 2024 Oct 29;10(10):CD002115. doi: 10.1002/14651858.CD002115.pub6. Cochrane Database Syst Rev. 2024. PMID: 39470206

Cited by

• The Caenorhabditis elegans ARIP-4 DNA helicase couples mitochondrial surveillance to immune, detoxification, and antiviral pathways.
  Mao K, Breen P, Ruvkun G. Mao K, et al. Proc Natl Acad Sci U S A. 2022 Dec 6;119(49):e2215966119. doi: 10.1073/pnas.2215966119. Epub 2022 Nov 29. Proc Natl Acad Sci U S A. 2022. PMID: 36445965 Free PMC article.
• Primary and secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliate Paramecium tetraurelia.
  Carradec Q, Götz U, Arnaiz O, Pouch J, Simon M, Meyer E, Marker S. Carradec Q, et al. Nucleic Acids Res. 2015 Feb 18;43(3):1818-33. doi: 10.1093/nar/gku1331. Epub 2015 Jan 15. Nucleic Acids Res. 2015. PMID: 25593325 Free PMC article.
• Designer siRNAs to overcome the challenges from the RNAi pathway.
  Jayasena SD. Jayasena SD. J RNAi Gene Silencing. 2005 Nov 30;2(1):109-17. J RNAi Gene Silencing. 2005. PMID: 19771212 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group
  • Ovid Technologies, Inc.