Muscle cytokine mRNA changes after 2.5 h of cycling: influence of carbohydrate - PubMed (original) (raw)
Clinical Trial
. 2005 Aug;37(8):1283-90.
doi: 10.1249/01.mss.0000175054.99588.b1.
J Mark Davis, Dru A Henson, Sarah J Gross, Charles L Dumke, Alan C Utter, Debra M Vinci, James A Carson, Adrienne Brown, Steve R McAnulty, Lisa S McAnulty, N Travis Triplett
Affiliations
- PMID: 16118573
- DOI: 10.1249/01.mss.0000175054.99588.b1
Clinical Trial
Muscle cytokine mRNA changes after 2.5 h of cycling: influence of carbohydrate
David C Nieman et al. Med Sci Sports Exerc. 2005 Aug.
Abstract
Purpose: To study the effect of carbohydrate compared to placebo ingestion on plasma cytokines and muscle cytokine mRNA following 2.5 h of intensive cycling in 15 trained cyclists.
Methods: Fifteen trained cyclists cycled for 2.5 h at 60% Wmax on two occasions while receiving 4 mL.kg.15 min carbohydrate (6%) (CHO) or placebo (PLA) beverages in a randomized, counterbalanced design. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and 12 h postexercise and compared to samples taken from five cyclists who rested in the lab during the exercise sessions. Blood cell counts were determined, and plasma was analyzed for interleukin (IL)-6, IL-10, IL-1 receptor antagonist (ra), IL-8, cortisol, epinephrine, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of four cytokines, IL-6, IL-8, tumor necrosis factor (TNF) alpha, and IL-1beta, using real-time quantitative reverse transcriptase polymerase chain reaction.
Results: Plasma glucose and insulin were higher, and epinephrine, cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO versus PLA. Muscle glycogen content decreased 68% immediately postexercise and the pattern of change did not differ between CHO and PLA. Muscle IL-6, IL-8, TNF-alpha, but not IL-1beta mRNA increased immediately postexercise compared to controls, with no differences between CHO and PLA.
Conclusion: CHO compared to PLA beverage ingestion attenuated the increase in plasma cortisol, epinephrine, IL-6, IL-10, and IL-1ra, but not muscle IL-6, IL-8, and TNF-alpha mRNA in athletes cycling 2.5 h at 60% Wmax.
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