Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells - PubMed (original) (raw)
. 2005 Aug 26;309(5739):1369-73.
doi: 10.1126/science.1116447.
Affiliations
- PMID: 16123299
- DOI: 10.1126/science.1116447
Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells
Chad A Cowan et al. Science. 2005.
Abstract
We have explored the use of embryonic stem cells as an alternative to oocytes for reprogramming human somatic nuclei. Human embryonic stem (hES) cells were fused with human fibroblasts, resulting in hybrid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antigen expression patterns characteristic of hES cells. Differentiation of hybrid cells in vitro and in vivo yielded cell types from each embryonic germ layer. Analysis of genome-wide transcriptional activity, reporter gene activation, allele-specific gene expression, and DNA methylation showed that the somatic genome was reprogrammed to an embryonic state. These results establish that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms.
Comment in
- Nuclear reprogramming by human embryonic stem cells.
Surani MA. Surani MA. Cell. 2005 Sep 9;122(5):653-4. doi: 10.1016/j.cell.2005.08.023. Cell. 2005. PMID: 16143098 Review.
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