E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer - PubMed (original) (raw)
doi: 10.1038/nsmb984. Epub 2005 Sep 4.
Affiliations
- PMID: 16142244
- DOI: 10.1038/nsmb984
E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer
Ziad M Eletr et al. Nat Struct Mol Biol. 2005 Oct.
Abstract
During ubiquitin ligation, an E2 conjugating enzyme receives ubiquitin from an E1 enzyme and then interacts with an E3 ligase to modify substrates. Competitive binding experiments with three human E2-E3 protein pairs show that the binding of E1s and of E3s to E2s are mutually exclusive. These results imply that polyubiquitination requires recycling of E2 for addition of successive ubiquitins to substrate.
Similar articles
- Binding to E1 and E3 is mutually exclusive for the human autophagy E2 Atg3.
Qiu Y, Hofmann K, Coats JE, Schulman BA, Kaiser SE. Qiu Y, et al. Protein Sci. 2013 Dec;22(12):1691-7. doi: 10.1002/pro.2381. Protein Sci. 2013. PMID: 24186333 Free PMC article. - Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating Enzymes and E3 Ubiquitin Ligases.
Brillada C, Trujillo M. Brillada C, et al. Methods Mol Biol. 2023;2581:13-29. doi: 10.1007/978-1-0716-2784-6_2. Methods Mol Biol. 2023. PMID: 36413307 - Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging.
Jin J, Li X, Gygi SP, Harper JW. Jin J, et al. Nature. 2007 Jun 28;447(7148):1135-8. doi: 10.1038/nature05902. Nature. 2007. PMID: 17597759 - Enzymes of ubiquitination and deubiquitination.
Neutzner M, Neutzner A. Neutzner M, et al. Essays Biochem. 2012;52:37-50. doi: 10.1042/bse0520037. Essays Biochem. 2012. PMID: 22708562 Review. - The 'ubiquitous' reality of vector immunology.
Severo MS, Sakhon OS, Choy A, Stephens KD, Pedra JH. Severo MS, et al. Cell Microbiol. 2013 Jul;15(7):1070-8. doi: 10.1111/cmi.12128. Epub 2013 Mar 14. Cell Microbiol. 2013. PMID: 23433059 Free PMC article. Review.
Cited by
- Computational design of second-site suppressor mutations at protein-protein interfaces.
Sammond DW, Eletr ZM, Purbeck C, Kuhlman B. Sammond DW, et al. Proteins. 2010 Mar;78(4):1055-65. doi: 10.1002/prot.22631. Proteins. 2010. PMID: 19899154 Free PMC article. - Auto-ubiquitination of Mdm2 enhances its substrate ubiquitin ligase activity.
Ranaweera RS, Yang X. Ranaweera RS, et al. J Biol Chem. 2013 Jun 28;288(26):18939-46. doi: 10.1074/jbc.M113.454470. Epub 2013 May 13. J Biol Chem. 2013. PMID: 23671280 Free PMC article. - The ubiquitin-proteasome system of Saccharomyces cerevisiae.
Finley D, Ulrich HD, Sommer T, Kaiser P. Finley D, et al. Genetics. 2012 Oct;192(2):319-60. doi: 10.1534/genetics.112.140467. Genetics. 2012. PMID: 23028185 Free PMC article. Review. - Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.
Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun F. Wang J, et al. J Biol Chem. 2013 Nov 22;288(47):33667-33681. doi: 10.1074/jbc.M113.503292. Epub 2013 Oct 2. J Biol Chem. 2013. PMID: 24089522 Free PMC article. - Control of APC/C-dependent ubiquitin chain elongation by reversible phosphorylation.
Craney A, Kelly A, Jia L, Fedrigo I, Yu H, Rape M. Craney A, et al. Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):1540-5. doi: 10.1073/pnas.1522423113. Epub 2016 Jan 25. Proc Natl Acad Sci U S A. 2016. PMID: 26811472 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
- R21 GM070597/GM/NIGMS NIH HHS/United States
- R01 GM069530/GM/NIGMS NIH HHS/United States
- R01 GM073960/GM/NIGMS NIH HHS/United States
- R01GM69530/GM/NIGMS NIH HHS/United States
- P30CA21765/CA/NCI NIH HHS/United States
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases